ILF3 Antibody

Western Blot: ILF3 Antibody [NBP1-84056] - Analysis in human cell line MOLT-4.

Immunocytochemistry/Immunofluorescence: ILF3 Antibody [NBP1-84056] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli & mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056] - Analysis in human lymph node and pancreas tissues. Corresponding ILF3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056] - Staining of human lymph node shows strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ILF3 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIK
• Predicted Species: Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ILF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ILF3 Protein (NBP1-84056PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24098121)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ILF3 Antibody

• Double-stranded RNA-binding protein 76
• double-stranded RNA-binding protein, 76 kD
• DRBF
• DRBP76M-phase phosphoprotein 4
• dsRNA binding protein NFAR-2/MPP4
• interleukin enhancer binding factor 3, 90kDa
• interleukin enhancer-binding factor 3
• MPHOSPH4interleukin enhancer binding factor 3, 90kD
• MPP4MMP4
• NF90CBTF
• NFAR-1
• NFAR2
• NFARNF110
• NF-AT-90
• Nuclear factor associated with dsRNA
• Nuclear factor of activated T-cells 90 kDa
• nuclear factor of activated T-cells, 90 kD
• TCP110
• TCP80
• Translational control protein 80

Background

ILF3 encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ILF3 Antibody (NBP1-84056)(2)

Publications using NBP1-84056

• Habjan M, Hubel P, Lacerda L et al. Sequestration by IFIT1 Impairs Translation of 2O-unmethylated Capped RNA. PLoS Pathog 2013-01-01 [PMID: 24098121]
• Stadler C, Hjelmare M, Neumann B et al. Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy. J Proteomics 2012-04-03 [PMID: 22361696]

Bioinformatics

• Gene Symbol: ILF3