p53 Antibody - BSA Free

Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: p53 Antibody [NB200-171] - FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 used at 1:5,000 (0.2ug/ml). Detection: DAB

Flow Cytometry: p53 Antibody [NB200-171] - p53 in Jurkat Cells, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 NB200-171 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- anti-KLH control IgG; Red- anti-HDMX.

Chromatin Immunoprecipitation Sequencing: p53 Antibody [NB200-171] - Chromatin from MCF7 was immunoprecipitated with anti-p53 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody used at the indicated concentrations.

Flow Cytometry: p53 Antibody [NB200-171] - Baseline p53 expression in human lymphocytes. Image from verified customer review.

Immunoprecipitation: p53 Antibody [NB200-171] - Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody NB200-171 used for IP at 3 ug per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody NB100-2204. For blotting immunoprecipitated P53, NB200-171 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IP, ChIP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

p53 Antibody - BSA Free Summary

• Description: This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NBP1-19098, NBP1-19097, NB100-376, NB100-1803, NB100-224, NB100-61615, NB100-77315, NB500-160, NB100-381, NB100-381, NB100-596, NB200-347, NB100-97835, NBP1-06527, NBP1-06515
• Immunogen: The immunogen this antibody was made to, maps to a region between residues 50-100 of human tumor protein p53 using the SwissProt entry P04637 (GeneID 7157).
• Localization: Cytoplasmic and nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP53
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation Sequencing
  • Flow Cytometry 1.5 ug/10^6 cells in 150 ul
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ChIP-Seq 4 ug/30ug chromatin.
• Control:

  293 Whole Cell Lysate
  p53 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NBP1-19098, NBP1-19097, NB100-376, NB100-1803, NB100-224, NB100-61615, NB100-77315, NB500-160, NB100-381, NB100-381, NB100-596, NB200-347, NB100-97835, NBP1-06527, NBP1-06515

Alternate Names for p53 Antibody - BSA Free

• Antigen NY-CO-13
• BCC7
• FLJ92943
• LFS1
• LFS1TRP53
• p53 tumor suppressor
• p53
• P53cellular tumor antigen p53
• Phosphoprotein p53
• TP53
• transformation-related protein 53
• TRP53
• tumor protein p53
• Tumor suppressor p53

Background

The tumor suppressor protein p53 plays an important role in cancer development. The p53 protein is a transcription factor that acts as a checkpoint in the cell cycle, either preventing or initiating programmed cell death. Since cancer occurs as the malfunction in the proliferation of cells, disrupting p53 activity allows abnormal cells to multiply or allows a tumor's progression from benign to malignant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p53 Antibody (NB200-171)(13)

Publications using NB200-171

• Yan Q, Ding J, Khan SJ et Al. Erratum: DTX3L E3 ligase targets p53 for degradation at poly ADP-ribose polymerase-associated DNA damage sites iScience 2024-07-19 [PMID: 39055911]
• Seonhwa Kim, Kyeong-Bae Jeon, Hyo-Min Park, Jinju Kim, Chae-Min Lim, Do-Young Yoon Establishment and Characterization of Immortalized Human Dermal Papilla Cells Expressing Human Papillomavirus 16 E6/E7 Journal of Microbiology and Biotechnology 2024-03-28 [PMID: 37994116]
• Yan Q, Ding J, Khan SJ et al. DTX3L E3 ligase targets p53 for degradation at poly ADP-ribose polymerase-associated DNA damage sites iScience 2023-04-21 [PMID: 37096048] (WB, Human)
• Alexander KA, CotE A, Nguyen SC et al. p53 mediates target gene association with nuclear speckles for amplified RNA expression Molecular cell 2021-04-15 [PMID: 33823140]
• Wilkes MC, Siva K, Chen J et al. Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase Nat Commun 2020-07-03 [PMID: 32620751] (FLOW, Human)
  
  Details:
  Cell surface FC
• Wan J, Block S, Scribano CM et al. Mad1 destabilizes p53 by preventing PML from sequestering MDM2 Nature communications 2019-04-04 [PMID: 30948704] (WB, Human)
• Spurlock CF, Gass HM, Bryant CJ et al. Methotrexate-mediated inhibition of nuclear factor kB activation by distinct pathways in T cells and fibroblast-like synoviocytes Rheumatology (Oxford) 2014-08-12 [PMID: 25118313]
• Spurlock CF, Tossberg JT, Matlock BK et al. Methotrexate inhibits NF-kB activity via lincRNA-p21 induction Arthritis & rheumatology 2014-07-30 [PMID: 25077978]
• Spurlock CF 3rd, Tossberg JT, Fuchs HA, Olsen NJ, Aune TM. Methotrexate increases expression of cell cycle checkpoint genes via Jun-N-terminal kinase activation. Arthritis Rheum. 2011-12-19 [PMID: 22183962] (FLOW, Human)
• Shultz LD, Saito Y, Najima Y et al. Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD/SCID/IL2r gamma(null) humanized mice. Proc Natl Acad Sci U S A 2010-07-20 [PMID: 20615947]
• Shao L, Fujii H, Colmegna I et al. Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis. J Exp Med;206(6):1435-1449. 2009-01-01 [PMID: 19451263]
• Wang SP, Wang WL, Chang YL et al. p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug. Nat Cell Biol 2009-06-01 [PMID: 19448627]
• Tsai KW, Tseng HC, Lin WC. Two wobble-splicing events affect ING4 protein subnuclear localization and degradation. Exp Cell Res 2008-10-15 [PMID: 18775696]

Review for p53 Antibody (NB200-171) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 06/30/2014

Summary

• Application: Flow Cytometry
• Sample Tested: Human PBMC
• Species: Human

FAQs for p53 Antibody (NB200-171). (Showing 1 - 1 of 1 FAQs).

1. I would like to know how you set the lot number of these antibodies. When does the lot number change and what is the difference between lots. Is every lot being QC tested? Or dependent on the host animals (in polyclonal)?
   • The lot numbers for our products, including NB200-104 and NB200-171, change with every purification or production. So each lot number will refer to the particular purification batch (if the antibody is provided purified) or production batch (if the antibody is not purified). The antibodies are QC tested every lot to ensure specificity and reactivity.

Bioinformatics

• Gene Symbol: TP53
• Entrez:
  • 7157(Human)
• Uniprot:
  • P04637()