Anti-p53 Antibodies: Novus Biologicals

NB200-103

p53 Antibody (PAb 240) - BSA Free

(3 Reviews)

(45 Publications)

0.1 ml

NB200-103

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 Antibody [PAb 240] NB200-103 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on MCF7 cells with p53 [PAb 240] Antibody NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on Neuro2a cells with p53 Antibody [PAb 240] NB200-103 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Reactivity:

Hu, Mu, Rt, Ye, Xp(-)

Species Glossary

Reactivity: Hu, Mu, Rt, Ye, Xp(-)

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-103AF350
Alexa Fluor 405	NB200-103AF405
Alexa Fluor 488	NB200-103AF488
Alexa Fluor 532	NB200-103AF532
Alexa Fluor 594	NB200-103AF594
Alexa Fluor 647	NB200-103AF647
Alexa Fluor 700	NB200-103AF700
Alexa Fluor 750	NB200-103AF750
Alexa Fluor Plus 405	NB200-103AFP405
Alexa Fluor Plus 488	NB200-103AFP488
Alexa Fluor Plus 555	NB200-103AFP555
Alexa Fluor Plus 594	NB200-103AFP594
Alexa Fluor Plus 647	NB200-103AFP647
Alexa Fluor Plus 680	NB200-103AFP680
Allophycocyanin	NB200-103APC
Biotin	NB200-103B
CoraFluor 1	NB200-103CL1
DyLight 350	NB200-103UV
DyLight 405	NB200-103V
DyLight 488	NB200-103G
DyLight 550	NB200-103R
DyLight 594	NB200-103DL594
DyLight 650	NB200-103C
DyLight 680	NB200-103FR
DyLight 755	NB200-103IR
FITC	NB200-103F
HRP	NB200-103H
Janelia Fluor 525	NB200-103JF525
Janelia Fluor 549	NB200-103JF549
Janelia Fluor 646	NB200-103JF646
Janelia Fluor 669	NB200-103JF669
PE	NB200-103PE
PerCP	NB200-103PCP
mFluor Violet 450 SE	NB200-103MFV450
mFluor Violet 500 SE	NB200-103MFV500
mFluor Violet 610 SE	NB200-103MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB200-103SS
Azide and BSA Free	NBP2-80898

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AF1355

p53 Antibody [Unconjugated]

(1 Review)

(31 Publications)

100 μg

AF1355

HAF109). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of CEM human T-lymphoblastoid cell line and MCF-7 human breast cancer cell line were mock-treated (-) or exposed (+) to 10 Gy ionizing radiation (IR) and harvested after 1 hour. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of CEM human T-lymphoblastoid cell line and MCF-7 human breast cancer cell line were mock-treated (-) or exposed (+) to 10 Gy ionizing radiation (IR) and harvested after 1 hour. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR." class="big_lightbox">

<b>Biological Strategies Validation. </b>MCF-7 human breast cancer cell line treated with 300 nM camptothecin overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. p53/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) or control antibody (Catalog # <a class=

AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for p53 at approximately 59 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF017). A specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

$<b>Genetic Strategies Validation. </b>Overexpression of Survivin in glioma cells affects cell cycle and proliferation in a p53 dependent manner. a Western blot analysis showing cell lysates from U87-MG and U87-MGshp53 after transduction with Survivin and control vectors. The membranes were blotted with anti-p53 (53 kDa), anti-p53s15 (53 kDa), anti-p21waf/cip (21 kDa), anti-gamma H2AX (16 kDa), anti-cyclin D1 (37 kDa) and E (42 kDa singlets +50 kDa doublets) antibodies and the signals comparing control and Survivin cells were measured. The relative band density (fold increase) obtained from the densitometric analysis and normalized to the corresponding control is depicted. alpha -actin (42 kDa) was used as an internal loading control. b Representative BrdU-incorporation assays of U87-MG (left panel) and U87-MGshp53 cells (right panel) with overexpression of Survivin (bottom) compared to the empty vector-transduced controls (top). The overall amount of BrdU-positive fractions as well as the relative levels of BrdU+ and BrdU− cells in fractions with DNA contents of 2n, 4n, 8n and >8n are depicted. c Proliferation index of the U87-MG and U87-MGshp53 cells transduced with Survivin and empty vector controls. Depicted are mean values ± SD. **p < 0.01. d BrdU-incorporation in U87-MG and U87-MGshp53 cell fractions with DNA content >4n. Depicted are mean values ± SD. **p < 0.01. All data were collected 72 h after transduction of cells Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3932-y), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Normal human lung fibroblasts and A549 cells acquire a senescent phenotype after ZNF365 silencing. Senescence-associated beta galactosidase activity (SA-beta Gal) staining photomicrograph of normal human lung fibroblasts (A) and A549 epithelial cells (C) transfected with scrambled control sequences “SCRAM” or with specific siRNA sequences for ZNF365 “siZNF” (Scale bar 100 μm). (B,D), Percentage of positive SA-beta Gal staining. Western blot analysis of senescent markers in ZNF365 silenced normal human lung fibroblasts (E) and A549 cells (F); beta actin was used as the internal loading control. Densitometric analyses are shown. (B,D) graphics represent the mean ± SD of one experiment with two technical replicates and ten random bright microscopic fields counted for each replicate. *** p < 0.001 by a two-tailed Student’s t-test with Welch’s correction. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36139424), licensed under a CC-BY license. Not internally tested by R&D Systems.

GH is induced in different senescence models & is a SASP component. (a) Oncogene‐induced senescence. WB of hNCC infected with lentivirus‐expressing, constitutively activated mutant V‐12HRAS oncogene (lentiV‐12HRAS) or empty vector (lenti‐CMV) & analyzed 7 days later. (b) DNA damage‐induced senescence. WB of hNCC exposed to indicated doses of UVC light or left untreated (NT) & analyzed 6 days later. (c) Replicative senescence. WB of hNCC passaged until proliferation was significantly reduced. from Passage 8 (p.8) & Passage 61 (p.61) compared. (d) WB of senescence markers in hNCC treated with DMSO (control) or 50 μM etoposide for 48 h & analyzed 7 days after beginning treatment. (e) Representative images of hNCC treated with DMSO (Control) or with 50 μM etoposide for 48 h & stained for SA‐ beta ‐gal (blue) & GH (brown) 6 days after beginning treatment. Note that only senescent express GH. Scale bar = 20 μm. (f, g) hNCC infected with lentivirus expressing shScr as control or shGH, treated with 50 μM etoposide (Etop) or DMSO (C) for 48 h, & analyzed 6 days after beginning treatment. (f) WB of cell GH & senescent marker expression. (g) WB of secreted GH in culture medium (in duplicate). EM, empty medium. Medium concentration was equalized according to cell number in wells. Ponceau was used as a loading control. (h) WB of GH & senescence markers & (i) real‐time PCR of GH in human fibroblasts derived from indicated progeroid syndrome patients & healthy volunteers as controls (C). AT, ataxia telangiectasia; CS, Cockayne syndrome; CSB, Cockayne syndrome group B; HGPS, Hutchinson–Gilford progeroid syndrome; XP, xeroderma pigmentosa. ImageJ quantification of WBs is depicted in Figure S1A & S2A,B,D,E. In a–d, f, & g, representative blots from at least three independent experiments are shown. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38724466), licensed under a CC-BY license. Not internally tested by R&D Systems.

Chemotherapeutic drugs DOX and PTX increase WT1 levels in CAFs and enhance their ability to inhibit T cell proliferation. A Schematic overview of drug treatment, co-culture of CAFs and PBMCs followed by T cell proliferation assay. B Protein expression of WT1 and p53 in CAFs following DOX and PTX treatment was determined by Western blotting. GAPDH was used as a loading control. C Protein levels of STAT1, STAT3, PD-L1 levels. IDO release from DOX and PTX treated CAFs was determined by ELISA. D DOX treated CAFs were co-cultured with PBMCs and CFSE-labelled CD4+ and CD8+ T cell proliferation was determined by flow cytometry (E) PTX treated CAFs were co-cultured with PBMCs and CFSE-labelled CD4+ and CD8.+ T cell proliferation was determined by flow cytometry. Representative experiment is shown of n = 3 biological replicates. Data are mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40598415), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Agarose	GAF1355
Alexa Fluor 350	AF1355U-100UG
Alexa Fluor 405	AF1355V-100UG
Alexa Fluor 488	AF1355G-100UG
Alexa Fluor 532	AF1355X-100UG
Alexa Fluor 594	AF1355T-100UG
Alexa Fluor 647	AF1355R-100UG
Alexa Fluor 700	AF1355N-100UG
Alexa Fluor 750	AF1355S-100UG
Alexa Fluor Plus 405	AF1355AFP405-100UG
Alexa Fluor Plus 488	AF1355AFP488-100UG
Alexa Fluor Plus 555	AF1355AFP555-100UG
Alexa Fluor Plus 594	AF1355AFP594-100UG
Alexa Fluor Plus 647	AF1355AFP647-100UG
Alexa Fluor Plus 680	AF1355AFP680-100UG
Allophycocyanin	AF1355A
Biotin	BAF1355
CoraFluor 1	AF1355CL1
DyLight 350	AF1355D
DyLight 405	AF1355E
DyLight 488	AF1355K
DyLight 550	AF1355L
DyLight 594	AF1355M
DyLight 650	AF1355W
DyLight 680	AF1355Y
DyLight 755	AF1355Z
FITC	AF1355F
HRP	HAF1355
Janelia Fluor 525	AF1355JF525
Janelia Fluor 549	AF1355I
Janelia Fluor 646	AF1355J
Janelia Fluor 669	AF1355JF669
PE	AF1355P
PerCP	AF1355C
mFluor Violet 450 SE	AF1355MFV450
mFluor Violet 500 SE	AF1355MFV500
mFluor Violet 610 SE	AF1355MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	HAF1355
Carrier Free	AF1355-SP

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NB200-171

p53 Antibody - BSA Free

(1 Review)

(13 Publications)

100 ul

NB200-171

Flow Cytometry: p53 Antibody [NB200-171] - p53 in Jurkat Cells, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 NB200-171 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- anti-KLH control IgG; Red- anti-HDMX.

Chromatin Immunoprecipitation Sequencing: p53 Antibody [NB200-171] - Chromatin from MCF7 was immunoprecipitated with anti-p53 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody used at the indicated concentrations.

Flow Cytometry: p53 Antibody [NB200-171] - Baseline p53 expression in human lymphocytes. Image from verified customer review.

<b>Independent Antibodies Validation. </b>Immunoprecipitation: p53 Antibody [NB200-171] - Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody NB200-171 used for IP at 3 ug per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody NB100-2204. For blotting immunoprecipitated P53, NB200-171 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-171AF350
Alexa Fluor 405	NB200-171AF405
Alexa Fluor 488	NB200-171AF488
Alexa Fluor 532	NB200-171AF532
Alexa Fluor 594	NB200-171AF594
Alexa Fluor 647	NB200-171AF647
Alexa Fluor 700	NB200-171AF700
Alexa Fluor 750	NB200-171AF750
Alexa Fluor Plus 405	NB200-171AFP405
Alexa Fluor Plus 488	NB200-171AFP488
Alexa Fluor Plus 555	NB200-171AFP555
Alexa Fluor Plus 594	NB200-171AFP594
Alexa Fluor Plus 647	NB200-171AFP647
Alexa Fluor Plus 680	NB200-171AFP680
Allophycocyanin	NB200-171APC
Biotin	NB200-171B
CoraFluor 1	NB200-171CL1
DyLight 350	NB200-171UV
DyLight 405	NB200-171V
DyLight 488	NB200-171G
DyLight 550	NB200-171R
DyLight 594	NB200-171DL594
DyLight 650	NB200-171C
DyLight 680	NB200-171FR
DyLight 755	NB200-171IR
FITC	NB200-171F
HRP	NB200-171H
Janelia Fluor 525	NB200-171JF525
Janelia Fluor 549	NB200-171JF549
Janelia Fluor 646	NB200-171JF646
Janelia Fluor 669	NB200-171JF669
PE	NB200-171PE
PerCP	NB200-171PCP
mFluor Violet 450 SE	NB200-171MFV450
mFluor Violet 500 SE	NB200-171MFV500
mFluor Violet 610 SE	NB200-171MFV610

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NBP2-29453

p53 Antibody (BP53-12)

(1 Review)

(11 Publications)

0.1 mg

NBP2-29453

DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the astrocytes showing nuclear staining. Protocol available in COMET™ Panel Builder." class="big_lightbox">

p53 was detected in immersion fixed paraffin-embedded sections of human Colon Tumor using Mouse Anti-Human p53 Monoclonal Antibody (Catalog # NBP2-29453) at 12ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the astrocytes showing nuclear staining. Protocol available in COMET™ Panel Builder." alt="p53 was detected in immersion fixed paraffin-embedded sections of human Colon Tumor using Mouse Anti-Human p53 Monoclonal Antibody (Catalog # NBP2-29453) at 12ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the astrocytes showing nuclear staining. Protocol available in COMET™ Panel Builder." class="big_thumb" />

Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (BP53-12).

Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - p53 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using a 1 ug/mL mouse anti-p53 monoclonal (NBP2-29453, Novus Biologicals) for 1 hour at room temperature followed by the appropriate HRP polymer reagent, anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffin human colon carcinoma stained with p53 Ab (BP53-12).

Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.

Reactivity:

Hu, Ca, Ch, Ha, Pm, Mu(-), Rt(-)

Species Glossary

Reactivity: Hu, Ca, Ch, Ha, Pm, Mu(-), Rt(-)

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

ABX-107A

Alt Catalog# ABX-107A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33074AF350
Alexa Fluor 405	NBP2-33074AF405
Alexa Fluor 488	NBP2-33074AF488
Alexa Fluor 532	NBP2-33074AF532
Alexa Fluor 594	NBP2-33074AF594
Alexa Fluor 647	NBP2-33074AF647
Alexa Fluor 700	NBP2-33074AF700
Alexa Fluor 750	NBP2-33074AF750
Alexa Fluor Plus 405	NBP2-33074AFP405
Alexa Fluor Plus 488	NBP2-33074AFP488
Alexa Fluor Plus 555	NBP2-33074AFP555
Alexa Fluor Plus 594	NBP2-33074AFP594
Alexa Fluor Plus 647	NBP2-33074AFP647
Alexa Fluor Plus 680	NBP2-33074AFP680
Allophycocyanin	NBP2-33074APC
Biotin	NBP2-33074B
CoraFluor 1	NBP2-33074CL1
DyLight 350	NBP2-33074UV
DyLight 405	NBP2-33074V
DyLight 488	NBP2-33074G
DyLight 550	NBP2-33074R
DyLight 594	NBP2-33074DL594
DyLight 650	NBP2-33074C
DyLight 680	NBP2-33074FR
DyLight 755	NBP2-33074IR
FITC	NBP2-33074F
HRP	NBP2-33074H
Janelia Fluor 525	NBP2-33074JF525
Janelia Fluor 549	NBP2-33074JF549
Janelia Fluor 646	NBP2-33074JF646
Janelia Fluor 669	NBP2-33074JF669
PE	NBP2-33074PE
mFluor Violet 450 SE	NBP2-33074MFV450
mFluor Violet 500 SE	NBP2-33074MFV500
mFluor Violet 610 SE	NBP2-33074MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33074
IHC-Prediluted	NBP2-44976

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AF1043

p53 [p Ser15] Antibody [Unconjugated]

(14 Publications)

100 μg

AF1043

HAF008). A specific band for Phospho-p53 (S15) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference, duplicate lysates were probed with 1:5000 dilution Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of CEM human T-lymphoblastoid cell line untreated or exposed to 100 J/m<SUP>2</SUP>UV-C for the indicated time. PVDF membrane was probed with 0.2 µg/mL Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). A specific band for Phospho-p53 (S15) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference, duplicate lysates were probed with 1:5000 dilution Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of CEM human T-lymphoblastoid cell line untreated or exposed to 100 J/m²UV-C for the indicated time. PVDF membrane was probed with 0.2 µg/mL Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S15) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference, duplicate lysates were probed with 1:5000 dilution Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using Rat Anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>p53 phosphorylated at S15 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with UV light (left panel) or untreated (right panel) using Rabbit Anti-Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # <a class=

NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using Rat Anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_lightbox">

p53 phosphorylated at S15 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_thumb" />

CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_lightbox">

CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_thumb" />

The increase of HDAC9 was associated with bone and fat imbalance in bone aging. a Micro-CT analyses of bone mass of trabecular and cortical bone thickness in the femora of 2-month-old (young) and 16-month-old (aged) mice. Bone mineral density (BMD), trabecular bone volume (BV/TV), and cortical bone thickness (Ct.Th) were performed. Scale bar = 1 mm. b–d Immunofluorescent staining of OCN (b), PPAR-gamma (c), and TRAP (d) was performed in the bone marrow from young and aged mice, and the positive signals were quantitatively analyzed. Scale bar = 50 μm. e Expressions of the senescence-related proteins, p53 and p-p53, in the bone marrow from young and aged mice were examined by western blotting. f Expressions of the senescence-related proteins, p53 and p-p53, in BMMSCs from young and aged mice were examined by western blotting. g Alizarin Red staining was performed, and quantification of mineralized nodules was analyzed in young and aged BMMSCs. h Oil Red O staining was performed, and quantification of lipid droplet positive ratio areas was analyzed in young and aged BMMSCs. Scale bars = 100 μm. i, j Expression of HDAC9 (i) and acetylation sites of H3K, including H3K9, H3K18, and H4K16 (j), were examined by western blotting. The data are presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired two-tailed Student’s t test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.

Downregulation of HDAC9 rescued lineage differentiation imbalance and ameliorated senescence in aged BMMSCs. a Alizarin Red staining was performed, and osteogenesis-related proteins were detected by western blotting in aged BMMSCs transfected with HDAC9 siRNA. b Oil Red O staining was performed, and adipogenesis-related proteins were detected by western blotting in aged BMMSCs transfected with HDAC9 siRNA. c Alizarin Red staining was performed, and osteogenic-related proteins were detected by western blotting in young BMMSCs and young BMMSCs transfected with HDAC9 siRNA. d Oil Red O staining was performed, and adipogenic-related proteins were detected by western blotting in young BMMSCs and young BMMSCs transfected with HDAC9 siRNA. e, f Expressions of the senescence-related proteins p53 and p-p53 in BMMSCs cultured in vitro from aged mice (e) and young mice (f) were examined by western blotting. The data are presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, one-way analysis of variance (ANOVA) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1043U-100UG
Alexa Fluor 405	AF1043V-100UG
Alexa Fluor 488	AF1043G-100UG
Alexa Fluor 532	AF1043X-100UG
Alexa Fluor 594	AF1043T-100UG
Alexa Fluor 647	AF1043R-100UG
Alexa Fluor 700	AF1043N-100UG
Alexa Fluor 750	AF1043S-100UG
Alexa Fluor Plus 405	AF1043AFP405-100UG
Alexa Fluor Plus 488	AF1043AFP488-100UG
Alexa Fluor Plus 555	AF1043AFP555-100UG
Alexa Fluor Plus 594	AF1043AFP594-100UG
Alexa Fluor Plus 647	AF1043AFP647-100UG
Alexa Fluor Plus 680	AF1043AFP680-100UG
Allophycocyanin	AF1043A
Biotin	AF1043B
CoraFluor 1	AF1043CL1
DyLight 350	AF1043D
DyLight 405	AF1043E
DyLight 488	AF1043K
DyLight 550	AF1043L
DyLight 594	AF1043M
DyLight 650	AF1043W
DyLight 680	AF1043Y
DyLight 755	AF1043Z
FITC	AF1043F
HRP	AF1043H
Janelia Fluor 525	AF1043JF525
Janelia Fluor 549	AF1043I
Janelia Fluor 646	AF1043J
Janelia Fluor 669	AF1043JF669
PE	AF1043P
PerCP	AF1043C
mFluor Violet 450 SE	AF1043MFV450
mFluor Violet 500 SE	AF1043MFV500
mFluor Violet 610 SE	AF1043MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1043-SP

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NBP2-62555

p53 Antibody (PAb421) - Chimeric - Azide and BSA Free

(5 Publications)

0.2 mg

NBP2-62555

Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Reactivity:

Hu, Mu, Rt, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Rb

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-62555-0.025mg

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MAB1355

p53 Antibody (184721)

(12 Publications)

100 μg

MAB1355

HAF1355). A specific band for p53 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of MCF-7 human breast cancer cell line mock-treated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. Human p53 was immunoprecipitated using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355). PVDF membrane was probed with HRP-conjugated Anti-Human/Mouse/Rat Polyclonal Antibody (Catalog # <a class=

HAF1355). A specific band for p53 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line mock-treated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. Human p53 was immunoprecipitated using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355). PVDF membrane was probed with HRP-conjugated Anti-Human/Mouse/Rat Polyclonal Antibody (Catalog # HAF1355). A specific band for p53 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Immunoblot analysis of kidney lysates.(A) Blot membranes were incubated with the antibodies against Nrf2, p53 and 8-oxoguanine glycosylase alpha (OGG1 alpha ). The mean integrated optical density is related to actin for Nrf2 (B), p53 (C) and mature 8-oxoguanine glycosylase alpha (D) levels. Results are given as a mean ± S.E.M. KD4, KD6, KD8 – groups of Eker rats (Tsc2+/−) treated with HFKD for four, six or eight mo., respectively; ST – Eker rats fed with a standard diet; LE ST – wild-type Long Evans rats treated with a standard diet; LE KD – wild-type Long Evans rats treated with a ketogenic diet similarly to the KD6 group. *P < 0.05, **p < 0.01, ***p < 0.001 as compared to ST unless otherwise stated (horizontal line) for ANOVA with a Fisher post hoc test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26892894), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1355U-100UG
Alexa Fluor 405	FAB1355V-100UG
Alexa Fluor 488	FAB1355G-100UG
Alexa Fluor 532	FAB1355X-100UG
Alexa Fluor 594	FAB1355T-100UG
Alexa Fluor 647	FAB1355R-100UG
Alexa Fluor 700	FAB1355N-100UG
Alexa Fluor 750	FAB1355S-100UG
Alexa Fluor Plus 405	FAB1355AFP405-100UG
Alexa Fluor Plus 488	FAB1355AFP488-100UG
Alexa Fluor Plus 555	FAB1355AFP555-100UG
Alexa Fluor Plus 594	FAB1355AFP594-100UG
Alexa Fluor Plus 647	FAB1355AFP647-100UG
Alexa Fluor Plus 680	FAB1355AFP680-100UG
Allophycocyanin	FAB1355A
Biotin	FAB1355B
CoraFluor 1	FAB1355CL1
DyLight 350	FAB1355D
DyLight 405	FAB1355E
DyLight 488	FAB1355K
DyLight 550	FAB1355L
DyLight 594	FAB1355M
DyLight 650	FAB1355W
DyLight 680	FAB1355Y
DyLight 755	FAB1355Z
FITC	FAB1355F
HRP	FAB1355H
Janelia Fluor 525	FAB1355JF525
Janelia Fluor 549	FAB1355I
Janelia Fluor 646	FAB1355J
Janelia Fluor 669	FAB1355JF669
PE	FAB1355P
PerCP	FAB1355C
mFluor Violet 450 SE	FAB1355MFV450
mFluor Violet 500 SE	FAB1355MFV500
mFluor Violet 610 SE	FAB1355MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1355-SP

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NBP2-34495

p53 Antibody (CL2199) - BSA Free

(1 Publication)

0.1 ml

NBP2-34495

Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells, but not in normal mucosa.

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human lung cancer (squamous cell carcinoma) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skin shows moderate to strong nuclear positivity in a subset of epidermal cells.

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Simple Western lane view shows a specific band for P53 in 0.2 mg/ml of Hek293T lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Electropherogram image(s) of corresponding Simple Western lane view. p53 antibody was used at 1:50 dilution on Hek293T lysate(s).

Staining in A431 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in MCF7 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in U2OS cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in U251 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43954AF350
Alexa Fluor 405	NBP3-43954AF405
Alexa Fluor 488	NBP3-43954AF488
Alexa Fluor 532	NBP3-43954AF532
Alexa Fluor 594	NBP3-43954AF594
Alexa Fluor 647	NBP3-43954AF647
Alexa Fluor 700	NBP3-43954AF700
Alexa Fluor 750	NBP3-43954AF750
Alexa Fluor Plus 405	NBP3-43954AFP405
Alexa Fluor Plus 488	NBP3-43954AFP488
Alexa Fluor Plus 555	NBP3-43954AFP555
Alexa Fluor Plus 594	NBP3-43954AFP594
Alexa Fluor Plus 647	NBP3-43954AFP647
Alexa Fluor Plus 680	NBP3-43954AFP680
Allophycocyanin	NBP3-43954APC
Biotin	NBP3-43954B
CoraFluor 1	NBP3-43954CL1
DyLight 350	NBP3-43954UV
DyLight 405	NBP3-43954V
DyLight 488	NBP3-43954G
DyLight 550	NBP3-43954R
DyLight 594	NBP3-43954DL594
DyLight 650	NBP3-43954C
DyLight 680	NBP3-43954FR
DyLight 755	NBP3-43954IR
FITC	NBP3-43954F
HRP	NBP3-43954H
Janelia Fluor 525	NBP3-43954JF525
Janelia Fluor 549	NBP3-43954JF549
Janelia Fluor 646	NBP3-43954JF646
Janelia Fluor 669	NBP3-43954JF669
PE	NBP3-43954PE
PerCP	NBP3-43954PCP
mFluor Violet 450 SE	NBP3-43954MFV450
mFluor Violet 500 SE	NBP3-43954MFV500
mFluor Violet 610 SE	NBP3-43954MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43954
BSA Free	NBP2-34495-25ul

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NBP2-00723

p53 Antibody (OTI5E2)

(1 Review)

(1 Publication)

0.1 ml

NBP2-00723

Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-p53 mouse monoclonal antibody.

Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-p53 antibody, and then analyzed by flow cytometry.

Western Blot: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-p53.

Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-p53 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-p53 mouse monoclonal antibody.

Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - Analysis of Hela cells, using anti-p53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Simple Western: p53 Antibody (5E2) [NBP2-00723] - Simple Western lane view shows a specific band for p53 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73200AF350
Alexa Fluor 405	NBP2-73200AF405
Alexa Fluor 488	NBP2-73200AF488
Alexa Fluor 532	NBP2-73200AF532
Alexa Fluor 594	NBP2-73200AF594
Alexa Fluor 647	NBP2-73200AF647
Alexa Fluor 700	NBP2-73200AF700
Alexa Fluor 750	NBP2-73200AF750
Alexa Fluor Plus 405	NBP2-73200AFP405
Alexa Fluor Plus 488	NBP2-73200AFP488
Alexa Fluor Plus 555	NBP2-73200AFP555
Alexa Fluor Plus 594	NBP2-73200AFP594
Alexa Fluor Plus 647	NBP2-73200AFP647
Alexa Fluor Plus 680	NBP2-73200AFP680
Allophycocyanin	NBP2-73200APC
Biotin	NBP2-73200B
CoraFluor 1	NBP2-73200CL1
DyLight 350	NBP2-73200UV
DyLight 405	NBP2-73200V
DyLight 488	NBP2-73200G
DyLight 550	NBP2-73200R
DyLight 594	NBP2-73200DL594
DyLight 650	NBP2-73200C
DyLight 680	NBP2-73200FR
DyLight 755	NBP2-73200IR
FITC	NBP2-73200F
HRP	NBP2-73200H
Janelia Fluor 525	NBP2-73200JF525
Janelia Fluor 549	NBP2-73200JF549
Janelia Fluor 646	NBP2-73200JF646
Janelia Fluor 669	NBP2-73200JF669
PE	NBP2-73200PE
PerCP	NBP2-73200PCP
mFluor Violet 450 SE	NBP2-73200MFV450
mFluor Violet 500 SE	NBP2-73200MFV500
mFluor Violet 610 SE	NBP2-73200MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73200

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NBP2-34387

p53 Antibody (SPM590)

(2 Publications)

0.1 mg

NBP2-34387

Reactivity:

Hu, Bv, Pm

Species Glossary

Reactivity: Hu, Bv, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-34434AF350
Alexa Fluor 405	NBP2-34434AF405
Alexa Fluor 488	NBP2-34434AF488
Alexa Fluor 532	NBP2-34434AF532
Alexa Fluor 594	NBP2-34434AF594
Alexa Fluor 647	NBP2-34434AF647
Alexa Fluor 700	NBP2-34434AF700
Alexa Fluor 750	NBP2-34434AF750
Alexa Fluor Plus 488	NBP2-34434AFP488
Alexa Fluor Plus 555	NBP2-34434AFP555
Alexa Fluor Plus 594	NBP2-34434AFP594
Alexa Fluor Plus 647	NBP2-34434AFP647
Alexa Fluor Plus 680	NBP2-34434AFP680
Allophycocyanin	NBP2-34434APC
Biotin	NBP2-34434B
CoraFluor 1	NBP2-34434CL1
DyLight 350	NBP2-34434UV
DyLight 405	NBP2-34434V
DyLight 488	NBP2-34434G
DyLight 550	NBP2-34434R
DyLight 594	NBP2-34434DL594
DyLight 650	NBP2-34434C
DyLight 680	NBP2-34434FR
DyLight 755	NBP2-34434IR
FITC	NBP2-34434F
HRP	NBP2-34434H
Janelia Fluor 525	NBP2-34434JF525
Janelia Fluor 549	NBP2-34434JF549
Janelia Fluor 646	NBP2-34434JF646
Janelia Fluor 669	NBP2-34434JF669
PE	NBP2-34434PE
PerCP	NBP2-34434PCP
mFluor Violet 450 SE	NBP2-34434MFV450
mFluor Violet 500 SE	NBP2-34434MFV500
mFluor Violet 610 SE	NBP2-34434MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-44979
Azide and BSA Free	NBP2-34434-0.1mg

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NBP2-19667

p53 Antibody - BSA Free

(1 Review)

0.1 ml

NBP2-19667

U373 xenograft, using p53 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.

Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-34308

p53 Antibody (DO-7)

(1 Publication)

0.1 mg

NBP2-34308

Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.

Reactivity:

Hu, Bv, Pm

Species Glossary

Reactivity: Hu, Bv, Pm

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-34605AF350
Alexa Fluor 405	NBP2-34605AF405
Alexa Fluor 488	NBP2-34605AF488
Alexa Fluor 532	NBP2-34605AF532
Alexa Fluor 594	NBP2-34605AF594
Alexa Fluor 647	NBP2-34605AF647
Alexa Fluor 700	NBP2-34605AF700
Alexa Fluor 750	NBP2-34605AF750
Alexa Fluor Plus 405	NBP2-34605AFP405
Alexa Fluor Plus 488	NBP2-34605AFP488
Alexa Fluor Plus 555	NBP2-34605AFP555
Alexa Fluor Plus 594	NBP2-34605AFP594
Alexa Fluor Plus 647	NBP2-34605AFP647
Alexa Fluor Plus 680	NBP2-34605AFP680
Allophycocyanin	NBP2-34605APC
Biotin	NBP2-34605B
CoraFluor 1	NBP2-34605CL1
DyLight 350	NBP2-34605UV
DyLight 405	NBP2-34605V
DyLight 488	NBP2-34605G
DyLight 550	NBP2-34605R
DyLight 594	NBP2-34605DL594
DyLight 650	NBP2-34605C
DyLight 680	NBP2-34605FR
DyLight 755	NBP2-34605IR
FITC	NBP2-34605F
HRP	NBP2-34605H
Janelia Fluor 525	NBP2-34605JF525
Janelia Fluor 549	NBP2-34605JF549
Janelia Fluor 646	NBP2-34605JF646
Janelia Fluor 669	NBP2-34605JF669
PE	NBP2-34605PE
mFluor Violet 450 SE	NBP2-34605MFV450
mFluor Violet 500 SE	NBP2-34605MFV500
mFluor Violet 610 SE	NBP2-34605MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-44978
Azide and BSA Free	NBP2-34605-0.1mg

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

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Host

Available Conjugates

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Species Glossary

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