P4HA1 Antibody (4F1) [DyLight 650]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

P4HA1 Antibody (4F1) [DyLight 650] Summary

• Immunogen: prolyl 4-hydroxylase, alpha polypeptide I - (Uniprot# P13674)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: P4HA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for P4HA1 Antibody (4F1) [DyLight 650]

• 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
• 2-oxoglutarate-4-dioxygenase subunit alpha-1
• 4-PH Alpha-1
• alpha polypeptide I
• EC 1.14.11.2
• P4HA
• P4HA1
• Procollagen-proline
• prolyl 4-hydroxylase, alpha polypeptide I

Background

P4HA1 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: P4HA1