p40/deltaNp63 Antibody (P40/8225R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with p40/deltaNp63 antibody (P40/8225R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human lung carcinoma stained with p40/deltaNp63 antibody (P40/8225R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: P40/8225R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

p40/deltaNp63 Antibody (P40/8225R) - Azide and BSA Free Summary

• Description: Positive Controls: HeLa cells. Human prostate or lung tissue (IHC).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa1-200) of human p40/deltaNp63 protein (exact sequence is proprietary)
• Localization: Nucleus.
• Specificity: The p40 (clone P40/8225R) antibody recognizes exclusively delta-Np63 but not TAp63 p40 is a squamous cell carcinoma specific antibody It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs benign prostate glands and for determining breast intraductal carcinoma vs invasive breast ductal carcinoma
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TP63
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for p40/deltaNp63 Antibody (P40/8225R) - Azide and BSA Free

• AIS
• amplified in squamous cell carcinoma
• B(p51A)
• B(p51B)
• Chronic ulcerative stomatitis protein
• EEC3
• Keratinocyte transcription factor KET
• KET
• LMS
• OFC8
• p53CP
• SHFM4
• TP53CP
• TP53L
• TP63
• TP73L
• Transformation-related protein 63
• tumor protein p53-competing protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP63