p40/deltaNp63 Antibody (P40/8225R) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: IHC
• Clone: P40/8225R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

p40/deltaNp63 Antibody (P40/8225R) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa1-200) of human p40/deltaNp63 protein (exact sequence is proprietary)
• Localization: Nucleus.
• Specificity: The p40 (clone P40/8225R) antibody recognizes exclusively delta-Np63 but not TAp63 p40 is a squamous cell carcinoma specific antibody It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs benign prostate glands and for determining breast intraductal carcinoma vs invasive breast ductal carcinoma
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TP63
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for p40/deltaNp63 Antibody (P40/8225R) [CoraFluor™ 1]

• AIS
• amplified in squamous cell carcinoma
• B(p51A)
• B(p51B)
• Chronic ulcerative stomatitis protein
• EEC3
• Keratinocyte transcription factor KET
• KET
• LMS
• OFC8
• p53CP
• SHFM4
• TP53CP
• TP53L
• TP63
• TP73L
• Transformation-related protein 63
• tumor protein p53-competing protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP63