P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free

Immunocytochemistry/Immunofluorescence: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Alignment of live-cell calcium response behavior with PTH abundance in a parathyroid adenoma. (A) Prestimulation baseline flux activity at time 0; (B) same field of view at 2 minutes after stimulation with 2 mM calcium; (C) same field after fixation and immunofluorescent staining for PTH (red); (D) image overlay of (B) and (C). Flux images (A, B) are single timepoint frames from the same time-ordered image stack. Anti-PTH reactivity is visualized by an AlexaFluor555 secondary antibody. Magnification = 200X. Image using the Allophycocyanin format of this antibody.

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Analysis using the DyLight 488 conjugate of NB100-65392. Thrombin stimulated equine platelets (solid line) and unstimulated platelets (dashed line) labeled with anti-CD62P (Psel.KO.2.7) compared to equine platelets labeled with murine IgG-DyLight 488 isotype control (gray fill).

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets.

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets.

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Analysis using the DyLight 488 conjugate of NB100-65392. Differential CD62P (P-selectin) expression of equine platelets in response to varying agonist treatments (Control (1), Thrombin (2), Convulxin (3), Ionophore A23187 (4)). All samples were first gated on CD61 positive events.

• Reactivity: Hu, Mu, Rt, Bv, Eq, Fe, Gt, Sh
• Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: Psel.KO.2.7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free Summary

• Immunogen: P-selectin transfected 300.19 cells.
• Localization: Membrane; single pass type I membrane protein.
• Specificity: NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:50
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
• Application Notes: For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. IHC-Frozen use reported in literature (see Massaguer, A. et al, 2000). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 31237986).
• Control:

  P-Selectin/CD62P Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P-Selectin/CD62P Antibody (NB100-65392)(15)

Publications using NB100-65392

• Mastrogiacomo L, Ballagh R, Venegas-Pino DE et al. The Effects of Hyperglycemia on Early Endothelial Activation and the Initiation of Atherosclerosis The American journal of pathology Oct 12 2022 [PMID: 36243046]
• Horioka K, Tanaka H, Isozaki S et al. Rewarming from accidental hypothermia enhances whole blood clotting properties in a murine model Thromb. Res. Jul 10 2020 [PMID: 32683149] (IHC-P, Mouse)
• Horioka K, Tanaka H, Shotaro I et al. Hypothermia-induced activation of the splenic platelet pool as a risk factor for thrombotic disease in a mouse model J. Thromb. Haemost. Jun 25 2019 [PMID: 31237986] (IHC-P, Mouse)
• Serpa PBS, Brooks MB, Divers T et al. Pharmacokinetics and Pharmacodynamics of an Oral Formulation of Apixaban in Horses After Oral and Intravenous Administration Front Vet Sci Dec 4 2018 [PMID: 30564584] (Flow, Equine)
• Stokol T, Serpa PBS, Brooks MB et al. Subcutaneous Administration of Low-Molecular-Weight Heparin to Horses Inhibits Ex Vivo Equine Herpesvirus Type 1-Induced Platelet Activation Front Vet Sci May 28 2018 [PMID: 29892605] (Flow, Equine)
• Hardy AT, Palma-Barqueros V, Watson SK et al. Significant Hypo-Responsiveness to GPVI and CLEC-2 Agonists in Pre-Term and Full-Term Neonatal Platelets and following Immune Thrombocytopenia Thromb. Haemost. Jun 1 2018 [PMID: 29695020] (Flow, Human)
• Samson AL, Alwis I, Maclean JAA et al. Endogenous fibrinolysis facilitates clot retraction in vivo. Blood. 2017 Oct 26 [PMID: 29074499] (Mouse)
• Stokol T, Serpa PB, Zahid MN, Brooks MB. Unfractionated and Low-Molecular-Weight Heparin and the Phosphodiesterase Inhibitors, IBMX and Cilostazol, Block Ex Vivo Equid Herpesvirus Type-1-Induced Platelet Activation. Front Vet Sci. Dec 2 2016 [PMID: 27909693] (FLOW, Human)
• Stokol T, Yeo WM, Burnett D et al. Equid herpesvirus type 1 activates platelets. PLoS ONE 2015 Apr 24 [PMID: 25905776] (FLOW, Equine)
  
  Details:
  Using the Allophycocyanin conjugated version of NB100-65392, catalog number NB100-65392APC.
• Watts AE, Ness SL, Divers TJ et al. Effects of clopidogrel on horses with experimentally induced endotoxemia. Am. J. Vet. Res. 1900 Jan 08 [PMID: 25061708]
• Brooks MB, Divers TJ, Watts AE et al. Effects of clopidogrel on the platelet activation response in horses. Am J Vet Res 2013 Sep [PMID: 23977894]
  
  Details:
  Using the DyLight 488 conjugated version of NB100-65392, catalog number NB100-65392G.
• Shirasuna K, Jiemtaweeboon S, Raddatz S et al. Rapid accumulation of polymorphonuclear neutrophils in the Corpus luteum during prostaglandin F(2alpha)-induced luteolysis in the cow. PLoS One. 2012 [PMID: 22235260]
• Johnson CA Jr, Wearden PD, Kocyildirim E et al. Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device. Artif Organs. 2011 Jun. [PMID: 21463346]
• Massaguer A, Engel P, Tovar V et al. Characterization of platelet and soluble-porcine P-selectin (CD62P). Vet Immunol Immunopathol. 2003 Dec. [PMID: 14592730]
• Massaguer A, Engel P, Perez-del-Pulgar S et al. Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P). Tissue Antigens. 2000 Aug. [PMID: 11019911]

FAQs for P-Selectin/CD62P Antibody (NB100-65392). (Showing 1 - 3 of 3 FAQs).

1. Does this antibody come conjugated to anything other than PE?
   • NB100-65392, clone Psel.KO.2.7 is only available conjugated to PE. We do sell kits that allow you to conjugate it to another color of your choice if you are interested. We can also do the conjugation in house for an additional fee and this can usually be done in 5-7 business days, depending on how busy the lab is. PE is our only readily available conjugation without an additional fee though.
2. I am considering purchasing NB100-65392. Do you have a recommended isotype control to use in conjunction?
   • NBP1-43778 is an appropriate isotype control.
3. The datasheet states that it is an IgG1 antibody. Is this IgG1 kappa or IgG1 lambda?
   • It is IgG1 kappa.

Bioinformatics

• Gene Symbol: SELP
• Entrez:
  • 6403(Human)
• Uniprot:
  • P16109()