PSGL-1/CD162 Antibody (HECA-452)

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Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using ...read more
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Human tonsil tissue stained with PSGL-1/CD162 Antibody (HECA-452) at 5ug/mL, and Leica Refine DAB kit. IHC-P image submitted by a verified ...read more
Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD3+ hPBMCs with Cutaneous Lymphocyte ...read more
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - IHC analysis of formalin-fixed paraffin-embedded tissue section of ...read more
Flow Cytometry: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Human peripheral blood lymphocytes stained with purified HECA-452, followed by ...read more
Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD14+ hPBMCs with Cutaneous Lymphocyte ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
HECA-452
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Catalog# & Formulation Size Price

PSGL-1/CD162 Antibody (HECA-452) Summary

Immunogen
Human tonsil stroma
Specificity
CLA antibody clone HECA-452 is known to recognize the cutaneous lymphocyte-associated antigen / CLA which is a specialized glycosylated form of P-selectin glycoprotein ligand-1/PSGL-1. Besides lymphocytes, this clone has been shown to react with normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241).
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Rat
Gene
SELPLG
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow (Cell Surface)
  • Flow Cytometry < = 2 ug/10^6 cells in 100 ul
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 1693467)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 2 ug/ml
Control
SR-BI Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-78039 in the following applications:

Publications
Read Publications using
NB100-78039 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for PSGL-1/CD162 Antibody (HECA-452)

  • CD162 antigen
  • CD162
  • P-selectin glycoprotein ligand 1
  • PSGL1
  • PSGL-1
  • PSGL-1cutaneous lymphocyte-associated associated antigen
  • selectin P ligandCLA
  • SELPLG

Background

Cutaneous Lymphocyte Antigen (CLA) is a single-pass type I membrane homodimer protein (140kDa) that collects in normal and inflamed skin. It is expressed on T lymphocytes, subsets of peripheral blood memory T cells, Natural Killer cells, memory B cells and dendritic cells, as well as on monocytes and granulocytes. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1). CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in leukocyte tethering and rolling over vascular surfaces during inflammation. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is appropriate for staining formalin-fixed paraffin embedded tissue sections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSGL-1/CD162 Antibody (NB100-78039)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: B/N, ELISA, FLOW, IF/IHC, WB.


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B/N
(1)
ELISA
(1)
FLOW
(3)
IF/IHC
(2)
WB
(3)
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Mouse
(2)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-78039 Applications Species
Li GX, Jiang XH, Zang JN et al. B-cell receptor associated protein 31 deficiency decreases the expression of adhesion molecule CD11b/CD18 and PSGL-1 in neutrophils to ameliorate acute lung injury The International Journal of Biochemistry & Cell Biology 2022-11-01 [PMID: 36210579] (WB, B/N) WB, B/N
Shi H, Jiang C, Yao H et al. CD44 fucosylation on bone marrow-derived mesenchymal stem cells enhances homing and promotes enteric nervous system remodeling in diabetic mice Cell & Bioscience 2021-12-01 [PMID: 34193268] (Mouse) Mouse
Yeini E, Ofek P, Pozzi S et al. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression Nature communications 2021-03-26 [PMID: 33771989] (FLOW) FLOW
Kim CH, Cheong KA, Park CD, Lee AY. Therapeutic Effects of Combination Using Glucosamine Plus Tacrolimus (FK-506) on The Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice. Scand J Immunol;75(5):471-8. 2012-05-01 [PMID: 22023698] (IF/IHC, Mouse) IF/IHC Mouse
Yamanaka K, Dimitroff CJ, Fuhlbrigge RC et al. Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression. J Allergy Clin Immunol. 2008-01-01 [PMID: 17910894] (WB) WB
Walcheck B, Leppanen A, Cummings R et al. The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin. Blood. 2002-06-01 [PMID: 12010808] (FLOW, ELISA) FLOW, ELISA
Borges E, Pendl G, Eytner R et al. The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated. J Biol Chem. 1997-11-01 [PMID: 9353350]
Duijvestijn AM, Horst E, Pals ST et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am J Pathol. 1988-01-01 [PMID: 3276207]
Berg EL, Yoshino T, Rott LS et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J Exp Med. 1991-12-01 [PMID: 1720810]
Picker LJ, Kishimoto TK, Smith CW et al. ELAM-1 is an adhesion molecule for skin-homing T cells. Nature. 1991-02-01 [PMID: 1705666]
Show All 11 Publications.

Review for PSGL-1/CD162 Antibody (NB100-78039) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-78039:
Filter by Applications
IHC-P
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin PSGL-1/CD162 NB100-78039
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5
reviewed by:
Verified Customer
IHC-P Human 10/13/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
LotB

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSGL-1/CD162 Antibody (NB100-78039) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Verified Customer
10/13/2021
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SELPLG