PSGL-1/CD162 Antibody (HECA-452)

Images

 
Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using ...read more
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Human tonsil tissue stained with PSGL-1/CD162 Antibody (HECA-452) at 5ug/mL, and Leica Refine DAB kit. IHC-P image submitted by a verified ...read more
Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD3+ hPBMCs with Cutaneous Lymphocyte ...read more
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - IHC analysis of formalin-fixed paraffin-embedded tissue section of ...read more
Flow Cytometry: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Human peripheral blood lymphocytes stained with purified HECA-452, followed by ...read more
Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD14+ hPBMCs with Cutaneous Lymphocyte ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
HECA-452
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Formulation Size Price

PSGL-1/CD162 Antibody (HECA-452) Summary

Immunogen
Human tonsil stroma
Specificity
Cutaneous Lymphocyte Antigen
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Rat
Gene
SELPLG
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow (Cell Surface)
  • Flow Cytometry < = 2 ug/10^6 cells in 100 ul
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 2 ug/ml
Application Notes
This antibody is Cytof ready. Use in ICC/IF was reported in scientific literature (PMID: 1693467).
Control
SR-BI Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-78039 in the following applications:

Publications
Read Publications using
NB100-78039 in the following applications:

  • 1 publication
  • 2 publications
  • 1 publication
  • IHC
    2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Notes

CLA antibody clone HECA-452 is known to recognize the cutaneous lymphocyte-associated antigen / CLA which is a specialized glycosylated form of P-selectin glycoprotein ligand-1/PSGL-1. Besides lymphocytes, this clone has been shown to react with normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241).

Alternate Names for PSGL-1/CD162 Antibody (HECA-452)

  • CD162 antigen
  • CD162
  • P-selectin glycoprotein ligand 1
  • PSGL1
  • PSGL-1
  • PSGL-1cutaneous lymphocyte-associated associated antigen
  • selectin P ligandCLA
  • SELPLG

Background

Cutaneous Lymphocyte Antigen (CLA) is a single-pass type I membrane homodimer protein (140kDa) that collects in normal and inflamed skin. It is expressed on T lymphocytes, subsets of peripheral blood memory T cells, Natural Killer cells, memory B cells and dendritic cells, as well as on monocytes and granulocytes. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1). CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in leukocyte tethering and rolling over vascular surfaces during inflammation. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is appropriate for staining formalin-fixed paraffin embedded tissue sections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSGL-1/CD162 Antibody (NB100-78039)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: ELISA, FLOW, Flow, IHC, WB.


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ELISA
(1)
FLOW
(2)
Flow
(1)
IHC
(2)
WB
(2)
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Mouse
(2)
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Showing Publications 1 - 10 of 10.
Publications using NB100-78039 Applications Species
Shi H, Jiang C, Yao H et al. CD44 fucosylation on bone marrow-derived mesenchymal stem cells enhances homing and promotes enteric nervous system remodeling in diabetic mice Cell & Bioscience Dec 1 2021 [PMID: 34193268] (Mouse) Mouse
Yeini E, Ofek P, Pozzi S et al. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression Nature communications Mar 26 2021 [PMID: 33771989] (Flow) Flow
Kim CH, Cheong KA, Park CD, Lee AY. Therapeutic Effects of Combination Using Glucosamine Plus Tacrolimus (FK-506) on The Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice. Scand J Immunol;75(5):471-8. 2012 May. [PMID: 22023698] (IHC, Mouse) IHC Mouse
Yamanaka K, Dimitroff CJ, Fuhlbrigge RC et al. Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression. J Allergy Clin Immunol. 2008 Jan [PMID: 17910894] (WB) WB
Walcheck B, Leppanen A, Cummings R et al. The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin. Blood. 2002 Jun 1 [PMID: 12010808] (ELISA, FLOW) ELISA, FLOW
Borges E, Pendl G, Eytner R et al. The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated. J Biol Chem. 1997 Nov. [PMID: 9353350]
Duijvestijn AM, Horst E, Pals ST et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am J Pathol. 1988 Jan. [PMID: 3276207]
Berg EL, Yoshino T, Rott LS et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J Exp Med. 1991 Dec. [PMID: 1720810]
Picker LJ, Kishimoto TK, Smith CW et al. ELAM-1 is an adhesion molecule for skin-homing T cells. Nature. 1991 Feb. [PMID: 1705666]
Picker LJ, Michie SA, Rott LS, Butcher EC. A unique phenotype of skin-associated lymphocytes in humans. Preferential expression of the HECA-452 epitope by benign and malignant T cells at cutaneous sites. Am J Pathol. 1990 May [PMID: 1693467] (FLOW, WB, IHC) FLOW, WB, IHC

Review for PSGL-1/CD162 Antibody (NB100-78039) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-78039:
Filter by Applications
IHC-P
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin PSGL-1/CD162 NB100-78039
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5
reviewed by:
Verified Customer
IHC-P Human 10/13/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
LotB

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSGL-1/CD162 Antibody (NB100-78039) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Verified Customer
10/13/2021
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SELPLG