P-Selectin/CD62P Antibody (Psel.KO.2.7) [Allophycocyanin]

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, IP
• Clone: Psel.KO.2.7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

P-Selectin/CD62P Antibody (Psel.KO.2.7) [Allophycocyanin] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: P-selectin transfected 300.19 cells
• Localization: Membrane; single pass type I membrane protein.
• Specificity: NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB100-65392. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Predicted cross-reactivities: Mouse, Equine, Goat, Bovine, Feline, Sheep, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for P-Selectin/CD62P Antibody (Psel.KO.2.7) [Allophycocyanin]

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P-Selectin/CD62P Antibody (NB100-65392APC)(1)

Publication using NB100-65392APC

• Stokol T, Yeo WM, Burnett D et al. Equid herpesvirus type 1 activates platelets. PLoS ONE 2015-04-24 [PMID: 25905776] (FLOW, Equine)
  
  Details:
  Using the Allophycocyanin conjugated version of NB100-65392, catalog number NB100-65392APC.

Review for P-Selectin/CD62P Antibody (NB100-65392APC) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer Flow Other 06/17/2015

Summary

• Application: Flow Cytometry
• Species: Other

Bioinformatics

• Gene Symbol: SELP