OXPAT Antibody - BSA Free

Western Blot: OXPAT Antibody [NB110-60511] - Detection of OXPAT in human (lane 1) and mouse (lane 2) heart lysate.

Simple Western: OXPAT Antibody [NB110-60511] - Simple Western lane view shows a specific band for OXPAT in 0.5 mg/ml of Human Heart (left) and Mouse Heart (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.01 mg/ml

OXPAT Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit OXPAT Antibody - BSA Free (NB110-60511) is a polyclonal antibody validated for use in WB and Simple Western. Anti-OXPAT Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a C-terminal region of the mouse OXPAT protein, within residues 400-463. [UniProt# Q8BVZ1]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLIN5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/ml
  • Western Blot 0.5-2 ug/ml
• Application Notes: In Western blot analysis, a band can be seen at approx. 50 kDa, representing the OXPAT protein.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Heart and Mouse Heart lysate 0.5 mg/mL, separated by Size, antibody dilution of 10 ug/mL. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  OXPAT Peptide (NB110-60511PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1.01 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OXPAT Antibody - BSA Free

• lipid storage droplet protein 5
• LSDA5
• LSDP5
• MLDP
• OXPAT
• perilipin 5
• perilipin-5

Background

OXPAT (PLIN5/Perilipin 5) is a novel PAT-1 (Perilipin, ADRP and TIP47) that binds to lipid droplets (LDs) and protects them from lipolytic degradation. The tissue distribution of PAT-1 proteins differs considerably, however, all of them are involved in accumulation and metabolism of lipids in LDs. OXPAT covers LDs in the cytosol and its expression is highest in tissues with a high fat-oxidative capacity, such as heart, oxidative (type I) skeletal muscles and brown adipose tissue wherein the OXPAT contents parallel the LD content. OXPAT overexpression in OP-9 and COS-7 cells increased cellular triglyceride storage in parallel with increases in fatty acid oxidation and induction of gene expression of mitochondrial enzymes involved in oxidative metabolism. OXPAT has the potential of modulating the interaction of the major triacylglycerol lipase ATGL with LD as well as with its co-factor CGI-58 in a well control manner to regulate LD lipolysis. OXPAT expression is up-regulated by PPARalpha, and this regulation appears to be part of an expression program that shifts the metabolism of cells from fatty acid storage to oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OXPAT Antibody (NB110-60511)(2)

Publications using NB110-60511

• Covington JD, Noland RC, Hebert RC et al. Perilipin 3 Differentially Regulates Skeletal Muscle Lipid Oxidation in Active, Sedentary and Type 2 Diabetic Males. J. Clin. Endocrinol. Metab. 2015-07-14 [PMID: 26171795]
• Wolins, NE et al. OXPAT/PAT-1 is a PPAR-induced lipid droplet protein that promotes fatty acid utilization. Diabetes;55(12):3418-28. 2006-12-01 [PMID: 17130488] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: PLIN5
• Entrez:
  • 440503(Human)
  • 66968(Mouse)