OXPAT Antibody [FITC]

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB110-60511F
    • Availability
      Product Discontinued

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OXPAT Antibody [FITC] Summary

Immunogen
A synthetic peptide made to a C-terminal region of the mouse OXPAT protein, within residues 400-463. [UniProt# Q8BVZ1]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
Application Notes
This OXPAT antibody is useful for Western blot analysis where a band can be seen at ~50 kDa, representing the OXPAT protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse. Immunogen sequence has 89% homology to rat and 75% homology to bovine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for OXPAT Antibody [FITC]

  • lipid storage droplet protein 5
  • LSDA5
  • LSDP5
  • MLDP
  • OXPAT
  • perilipin 5
  • perilipin-5

Background

OXPAT (PLIN5/Perilipin 5) is a novel PAT-1 (Perilipin, ADRP and TIP47) that binds to lipid droplets (LDs) and protects them from lipolytic degradation. The tissue distribution of PAT-1 proteins differs considerably, however, all of them are involved in accumulation and metabolism of lipids in LDs. OXPAT covers LDs in the cytosol and its expression is highest in tissues with a high fat-oxidative capacity, such as heart, oxidative (type I) skeletal muscles and brown adipose tissue wherein the OXPAT contents parallel the LD content. OXPAT overexpression in OP-9 and COS-7 cells increased cellular triglyceride storage in parallel with increases in fatty acid oxidation and induction of gene expression of mitochondrial enzymes involved in oxidative metabolism. OXPAT has the potential of modulating the interaction of the major triacylglycerol lipase ATGL with LD as well as with its co-factor CGI-58 in a well control manner to regulate LD lipolysis. OXPAT expression is up-regulated by PPARalpha, and this regulation appears to be part of an expression program that shifts the metabolism of cells from fatty acid storage to oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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OXPAT NB110-60511F

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Research Areas for OXPAT Antibody (NB110-60511F)

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Blogs on OXPAT.

OXPAT: Regulating Lipid Metabolism during Dietary Fluctuations
OXPAT is the mammalian form of the PAT (perilipin, adipophilin, and TIP47) gene family that consists of proteins associated with lipid droplets (LDs) in fat-storing adipocyte cells. They serve to protect LDs from natural lipases responsible for breaki...  Read full blog post.

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Bioinformatics

Gene Symbol PLIN5