Otx2 Antibody (PCRP-OTX2-1E10) - Azide and BSA Free

• Reactivity: Hu
• Applications: ELISA, Flow, IP
• Clone: PCRP-OTX2-1E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Otx2 Antibody (PCRP-OTX2-1E10) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13986).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant human full-lengthOtx2protein (Uniprot: P32243)
• Localization: Nucleus
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTX2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
• Application Notes: ELISA: For coating, order this antibody without BSA Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 71.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Chick, Mouse, Rat, Xenopus and Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Otx2 Antibody (PCRP-OTX2-1E10) - Azide and BSA Free

• homeobox protein OTX2
• MCOPS5
• MGC45000
• orthodenticle homeobox 2
• orthodenticle homolog 2 (Drosophila)
• Orthodenticle homolog 2
• Otx2

Background

Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTX2