Otx2 Antibody (1H12G8B2)

Western Blot: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of U251 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of colon tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.

Western Blot: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)

Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of stomach tissues (left) and brain tissues (right) using OTX2 mouse mAb with DAB staining.

ELISA: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1H12G8B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Otx2 Antibody (1H12G8B2) Summary

• Immunogen: Purified recombinant fragment of human Otx2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTX2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Otx2 Antibody (1H12G8B2)

• homeobox protein OTX2
• MCOPS5
• MGC45000
• orthodenticle homeobox 2
• orthodenticle homolog 2 (Drosophila)
• Orthodenticle homolog 2
• Otx2

Background

Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTX2