ORP150/HSP12A Antibody (6F7) - Azide and BSA Free

Western Blot: ORP150/HSP12A Antibody (6F7) [H00010525-M01] - HYOU1 monoclonal antibody (M01), clone 6F7 Analysis of HYOU1 expression in MCF-7.

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (6F7) [H00010525-M01] - Analysis of monoclonal antibody to HYOU1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ug/ml.

ELISA: ORP150/HSP12A Antibody (6F7) [H00010525-M01] - Detection limit for recombinant GST tagged HYOU1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, MA
• Clone: 6F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ORP150/HSP12A Antibody (6F7) - Azide and BSA Free Summary

• Immunogen: HYOU1 (NP_006380, 901 a.a. ~ 999 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVQYLLNKAKFTKPRPRPKDKNGTRAEPPLNASASDQGEKVIPPAGQTEDAEPISEPEKVETGSEPGDTEPLELGGPGAEPEQKEQSTGQKRPLKNDEL
• Specificity: HYOU1 - hypoxia up-regulated 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HYOU1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Microarray
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ORP150/HSP12A Antibody (6F7) - Azide and BSA Free

• 150 kDa oxygen-regulated protein
• 170 kDa glucose-regulated protein
• DKFZp686N08236
• FLJ97572
• glucose-regulated protein 170
• Grp170
• GRP-170
• HSP12A
• HYOU1
• hypoxia up-regulated 1
• hypoxia up-regulated protein 1
• ORP150
• ORP-150
• ORP150FLJ94899
• oxygen regulated protein (150kD)

Background

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ORP150/HSP12A Antibody (H00010525-M01)(3)

Publications using H00010525-M01

• Yoshida Y, Yamashita T, Nagano K et al. Limited expression of reticulocalbin-1 in lymphatic endothelial cells in lung tumor but not in normal lung. Biochem Biophys Res Commun. 2011-01-25 [PMID: 21272564]
• Lindenmeyer MT, Rastaldi MP, Ikehata M et al. Proteinuria and Hyperglycemia Induce Endoplasmic Reticulum Stress. J Am Soc Nephrol 2008-09-05 [PMID: 18776125]
• Liu R, Li Z, Bai S et al. Mechanism of cancer cell adaptation to metabolic stress: proteomics identification of a novel thyroid hormone mediated gastric carcinogenic signaling pathway. Mol Cell Proteomics;8(1):70-85. 2009-01-01 [PMID: 18723843]

Bioinformatics

• Gene Symbol: HYOU1
• Entrez:
  • 10525(Human)
• OMIM:
  • 601746(Human)
• Uniprot:
  • NP_006380(Human)