ORP150/HSP12A Antibody (6E3-2C3) - BSA Free

Western Blot: ORP150/HSP12A Antibody (6E3-2C3) [NBP2-59345] - Western Blot analysis of Rat Liver showing detection of ~170 kDa ORP150/HSP12A protein using Mouse Anti-ORP150/HSP12A Monoclonal Antibody, Clone 6E3-2C3 (NBP2-59345). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ORP150/HSP12A Monoclonal Antibody (NBP2-59345) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.

Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody (6E3-2C3) [NBP2-59345] - Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

Western Blot: ORP150/HSP12A Antibody (6E3-2C3) [NBP2-59345] - Analysis of Human HEK-293 cell lysate showing detection of ~170 kDa GRP170 protein using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6E3-2C2. Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK-293 cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 6E3-2C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

ORP150/HSP12A Antibody (6E3-2C3) - BSA Free Summary

• Immunogen: Recombinant Full length GRP170 Protein
• Localization: Endoplasmic Reticulum , Endoplasmic Reticulum Lumen
• Specificity: Detects 170 kDa.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HYOU1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Purity: Protein G purified

Alternate Names for ORP150/HSP12A Antibody (6E3-2C3) - BSA Free

• 150 kDa oxygen-regulated protein
• 170 kDa glucose-regulated protein
• DKFZp686N08236
• FLJ97572
• glucose-regulated protein 170
• Grp170
• GRP-170
• HSP12A
• HYOU1
• hypoxia up-regulated 1
• hypoxia up-regulated protein 1
• ORP150
• ORP-150
• ORP150FLJ94899
• oxygen regulated protein (150kD)

Background

HYOU1 is encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HYOU1