| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, Flow, IHC, IHC-P |
| Clone | ODC1/486 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 405 |
| Immunogen | Recombinant human ODC-1 protein |
| Localization | Cytoplasmic |
| Specificity | Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ODC1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![Ornithine Decarboxylase Antibody (ODC1/486) [DyLight 405]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Ornithine Decarboxylase Antibody (ODC1/486) [DyLight 405]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-47836AF350 | |
| Alexa Fluor 405 | NBP2-47836AF405 | |
| Alexa Fluor 488 | NBP2-47836AF488 | |
| Alexa Fluor 532 | NBP2-47836AF532 | |
| Alexa Fluor 594 | NBP2-47836AF594 | |
| Alexa Fluor 647 | NBP2-47836AF647 | |
| Alexa Fluor 700 | NBP2-47836AF700 | |
| Alexa Fluor 750 | NBP2-47836AF750 | |
| Allophycocyanin | NBP2-47836APC | |
| Biotin | NBP2-47836B | |
| DyLight 350 | NBP2-47836UV | |
| DyLight 405 | NBP2-47836V | |
| DyLight 488 | NBP2-47836G | |
| DyLight 550 | NBP2-47836R | |
| DyLight 594 | NBP2-47836DL594 | |
| DyLight 650 | NBP2-47836C | |
| DyLight 680 | NBP2-47836FR | |
| DyLight 755 | NBP2-47836IR | |
| FITC | NBP2-47836F | |
| HRP | NBP2-47836H | |
| Janelia Fluor 549 | NBP2-47836JF549 | |
| Janelia Fluor 646 | NBP2-47836JF646 | |
| PE | NBP2-47836PE | |
| PerCP | NBP2-47836PCP |
Diseases for Ornithine Decarboxylase Antibody (NBP2-47836V)Discover more about diseases related to Ornithine Decarboxylase Antibody (NBP2-47836V).
| Pathways for Ornithine Decarboxylase Antibody (NBP2-47836V)View related products by pathway.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ODC1 |
![Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY licence.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Western-Blot-NB600-302-img0022.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, clone 9E10) at 1:50 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Immunohistochemistry-Paraffin-NB600-302-img0009.jpg)
![Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Simple-Western-NB600-302-img0006.jpg)
![ELISA: AMD1 Antibody [NB100-75341] - Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/ml) and serial dilutions of antibody. ( anti-AMD1 )](http://images.novusbio.com/images/p_75341_1.jpg)
![Western Blot: Arginine decarboxylase Antibody [NBP2-15447] - A. 30 ug human normal cerebellar lysate/extract10 % SDS-PAGE ADC antibody dilution: 1:1500](http://images.novusbio.com/images2/Arginine-decarboxylase-Antibody-Western-Blot-NBP2-15447-img0008.jpg)
![Western Blot: Arginine decarboxylase Antibody [NBP2-15447] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/images2/Arginine decarboxylase Antibody-Western Blot-NBP2-15447-img0001.jpg)
![Western Blot: Arginine decarboxylase Antibody [NBP2-15447] - A. 30 ug SK-N-SH whole cell lysate/extract 10 % SDS-PAGE ADC antibody dilution: 1:5000](http://images.novusbio.com/images2/Arginine-decarboxylase-Antibody-Western-Blot-NBP2-15447-img0006.jpg)
![Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/c-Fos-Antibody-(2H2)-Knockout-Validated-NBP2-50037-img0005.jpg)
![Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Analysis of cell lysates using mouse c-Fos mAb, dilution 1:1,000 (Green), and rabbit GAPDH pAb, dilution 1:20,000 (Red) used as a loading control. [1] protein standard (red), [2] HeLa cells in serum free media. [3] HeLa cells stimulated with 20% fetal bovine serum for 2hrs after 36hrs in serum free media. [4] rat cortical neurons. [5] rat cortical neurons treated with membrane depolarization buffer for 5hrs. Multiple bands at 50-65kDa in stimulated or treated cell lysates correspond to different forms of the c-Fos proten. The single band at 37 kDa represents GAPDH protein.](http://images.novusbio.com/images2/c-Fos-Antibody-(2H2)-Western-Blot-NBP2-50037-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.](http://images.novusbio.com/images2/c-Fos-Antibody-(2H2)-Immunocytochemistry-Immunofluorescence-NBP2-50037-img0006.jpg)
![Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Western-Blot-NBP2-34260-img0006.jpg)
![Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-NBP2-34260-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Western-Blot-NBP2-37576-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.](http://images.novusbio.com/images2/Catalase-Antibody-Western-Blot-NBP2-24916-img0001.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/images2/Catalase-Antibody-Immunohistochemistry-Paraffin-NBP2-24916-img0002.jpg)
![Western Blot: SAT1 Antibody [NB110-41622] - Detection of SAT1 in human SAT1 transfected lysate.](http://images.novusbio.com/images2/SAT1 Antibody-Western Blot-NB110-41622-img0004.jpg)
![Immunohistochemistry: SAT1 Antibody [NB110-41622] - IHC analysis of SAT1 in mouse seminal vesical (left) and prostate (right) usind DAB with hematoxylin counterstain,](http://images.novusbio.com/images2/SAT1-Antibody-Immunohistochemistry-NB110-41622-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding QPCT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Immunohistochemistry-Paraffin-NBP1-81838-img0010.jpg)
![Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in control (vector only transfected HEK293T lysate) and QPCT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Western-Blot-NBP1-81838-img0011.jpg)
![Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Western-Blot-NBP1-81838-img0006.jpg)
![Western Blot: SRM Antibody [NBP1-80726] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/SRM-Antibody-Western-Blot-NBP1-80726-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SRM Antibody [NBP1-80726] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody Antibody staining is shown in green.](http://images.novusbio.com/images2/SRM-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80726-img0006.jpg)
![Immunohistochemistry-Paraffin: SRM Antibody [NBP1-80726] - Staining of human stomach shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/SRM-Antibody-Immunohistochemistry-Paraffin-NBP1-80726-img0002.jpg)
![Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours (right lane) and 10 uL of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/images2/iNOS-Antibody-Western-Blot-NB300-605-img0015.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.](http://images.novusbio.com/images2/iNOS-Antibody-Immunohistochemistry-Paraffin-NB300-605-img0010.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.](http://images.novusbio.com/images2/iNOS-Antibody-Immunohistochemistry-Paraffin-NB300-605-img0016.jpg)
![Western Blot: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.](http://images.novusbio.com/images2/DBT-Antibody-(OTI1G2)-Western-Blot-NBP2-02042-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DBT Antibody (OTI1G2) [NBP2-02042] - Anti-DBT mouse monoclonal antibody (NBP2-02042) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DBT.](http://images.novusbio.com/images2/DBT-Antibody-(OTI1G2)-Immunocytochemistry-Immunofluorescence-NBP2-02042-img0003.jpg)
![Flow Cytometry: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DBT antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images2/DBT-Antibody-(OTI1G2)-Flow-Cytometry-NBP2-02042-img0001.jpg)
![SDS-Page: Recombinant Human PRH2 Protein [H00005555-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00005555-P01-1.jpg)