Orc2 Antibody

Images

 
Western Blot: Orc2 Antibody [NBP1-46175] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody used ...read more
Immunohistochemistry: Orc2 Antibody [NBP1-46175] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ORC2 used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB staining using ...read more
Proximity Ligation Assay: Orc2 Antibody [NBP1-46175] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human ORC2 (NBP1-46175). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: Orc2 Antibody [NBP1-46175] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM7 (NB100-252) and rabbit anti-human ORC2 (NBP1-46175). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: Orc2 Antibody [NBP1-46175] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RPA2 (NBP2-22232) and rabbit anti-human ORC2 (NBP1-46175). Image merged from DAPI (2ms) and Texas ...read more

Product Details

Summary
Reactivity Hu, Eq, Mk, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Orc2 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1 and 50 of human Origin Recognition Complex, Subunit 2-like using the numbering given in entry NP_006181.1 (GeneID 4999).
Marker
Chromatin Marker
Predicted Species
Primate (100%), Equine (100%), Monkey (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
ORC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:200-1:4000

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque and Northern white cheeked gibbon.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Orc2 Antibody

  • ORC2Lorigin recognition complex subunit 2
  • origin recognition complex protein 2 homolog
  • origin recognition complex, subunit 2 (yeast homolog)-like
  • origin recognition complex, subunit 2 homolog (yeast)
  • origin recognition complex, subunit 2 homolog
  • origin recognition complex, subunit 2
  • origin recognition complex, subunit 2-like (yeast)
  • origin recognition complex, subunit 2-like

Background

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3L, -4L, and -5L. It also interacts with CDC45L and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ORC2

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