Orai2 Antibody - BSA Free

Western Blot: Orai2 Antibody [NBP1-77283] - Analysis of ORAI2 in Jurkat cell lysate with ORAI2 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.

Immunocytochemistry/Immunofluorescence: Orai2 Antibody [NBP1-77283] - ORAI2 in Jurkat cells with ORAI2 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: Orai2 Antibody - BSA Free [NBP1-77283] - Immunofluorescence of Orai2 in Jurkat cells with Orai2 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Ch
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Orai2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ORAI2. The immunogen is located within the last 50 amino acids of ORAI2.
• Specificity: At least two isoforms of ORAI2 are known to exist; this antibody will detect both isoforms. ORAI antibody is predicted not to cross-react with ORAI1 or ORAI3.
• Predicted Species: Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ORAI2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5-20 ug/ml
  • Western Blot 1-4 ug/ml
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Orai2 Peptide (NBP1-77283PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Orai2 Antibody - BSA Free

• C7orf19
• CAP-binding protein complex interacting protein 2
• CAP-binding protein complex-interacting protein 2
• CBCIP2
• CBCIP2C7orf19FLJ12474
• chromosome 7 open reading frame 19
• FLJ14733
• H_NH0514P08.8
• MEM142B
• ORAI calcium release-activated calcium modulator 2
• Orai2
• PP1729
• protein orai-2
• putative protein ORAI2-2
• TMEM142B
• Transmembrane protein 142BFLJ44818

Background

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ORAI2
• Uniprot:
  • Q96SN7(Human)