Orai2 Antibody

Images

 
Western Blot: Orai2 Antibody [NBP1-77283] - Analysis of ORAI2 in Jurkat cell lysate with ORAI2 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
Immunocytochemistry: Orai2 Antibody [NBP1-77283] - ORAI2 in Jurkat cells with ORAI2 antibody at 5 ug/mL.
Immunofluorescence: Orai2 Antibody [NBP1-77283] - ORAI2 in Jurkat cells with ORAI2 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, ChSpecies Glossary
Applications WB, ELISA, ICC, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Orai2 Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ORAI2. The immunogen is located within the last 50 amino acids of ORAI2.
Specificity
At least two isoforms of ORAI2 are known to exist; this antibody will detect both isoforms. ORAI antibody is predicted not to cross-react with ORAI1 or ORAI3.
Predicted Species
Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ORAI2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 4 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry 5 ug/ml
  • Immunofluorescence 20 ug/ml
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Orai2 Peptide (NBP1-77283PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Orai2 Antibody

  • CAP-binding protein complex interacting protein 2
  • CAP-binding protein complex-interacting protein 2
  • CBCIP2C7orf19FLJ12474
  • chromosome 7 open reading frame 19
  • FLJ14733
  • H_NH0514P08.8
  • MEM142B
  • ORAI calcium release-activated calcium modulator 2
  • protein orai-2
  • putative protein ORAI2-2
  • TMEM142B
  • Transmembrane protein 142BFLJ44818

Background

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ORAI2
Uniprot