Opsin 1 (Medium Wave) Antibody

Images

 
Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of rat eye. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further ...read more
Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IF analysis of Opsin 1 in paraffin embedded and frozen mouse retina tissues. Image courtesy of product review submitted by Linda Vuong.
Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of rat eye.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Opsin 1 (Medium Wave) Antibody Summary

Immunogen
A synthetic peptide as a part of mouse and rat OPSG (green-sensitive opsin, M opsin, Opn1mw, Gcp) conjugated to immunogenic carrier protein was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OPN1MW
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1: 1000 - 1 : 2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:300-1:2000
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB110-74730 in the following applications:

Publications
Read Publication using
NB110-74730 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for Opsin 1 (Medium Wave) Antibody

  • GCP
  • GOP
  • Green cone photoreceptor pigment
  • Green-sensitive opsin
  • medium-wave-sensitive opsin 1
  • OPN1MW
  • opsin 1 (cone pigments), medium-wave-sensitive 2

Background

FUNCTION: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in cone photoreceptor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-45057
Species: Hu, Mu, Rt, Po
Applications: WB, ICC/IF, IHC, IHC-P
AF1513
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
H00005956-R02
Species: Hu
MAB333
Species: Hu
Applications: WB, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-52546
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
NBP1-84495
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-236
Species: Hu, Mu
Applications: WB, IB, IHC, IHC-Fr, IHC-P, IP
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-00823
Species: Hu, Mu, Rt, Ca
Applications: WB, Flow, IHC, IHC-P
NBP1-84956
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-47602
Species: Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NB100-91761
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2436
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NB100-230
Species: Hu
Applications: WB, IP, PLA
NB600-600
Species: Hu, Mu
Applications: WB, ChIP, ELISA, IHC, IHC-P, Flow-IC
PP-B0422-00
Species: Hu, Mu
Applications: WB, IHC, IP, DirELISA
NBP2-20116
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-74730
Species: Mu, Rt
Applications: WB, IHC, IHC-P

Publications for Opsin 1 (Medium Wave) Antibody (NB110-74730)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for Opsin 1 (Medium Wave) Antibody (NB110-74730) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.
We have 3 reviews tested in 2 application: IHC-P, IHC.

Reviews using NB110-74730:
Filter by Applications
IHC-P
(1)
IHC
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
 Opsin 1 (Medium Wave) NB110-74730
Enlarge
reviewed by:
Henri Leinonen
Mouse 07/18/2017
View

Summary

Sample Testedmouse retina
SpeciesMouse
LotRB0216-251107

Comments

CommentsAnti-rabbit AlexaFluor 647 nm secondary antibody
 
reviewed by:
Anonymous
IHC-P Mouse 06/21/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse retina embedded in paraffin
SpeciesMouse
Immunohistochemistry Opsin 1 (Medium Wave) NB110-74730
Enlarge
reviewed by:
Anonymous
IHC Mouse 08/14/2012
View

Summary

ApplicationImmunohistochemistry
Sample TestedMouse retina embedded in paraffin and frozen medium
SpeciesMouse
Lot08062012
CommentsThe datasheet said that the antibody would work for western blots and IHC using paraffin sections. Our lab uses both paraffin and frozen sections for IHC, so we tested the antibody on both types of sections. We got comparable results using the antibody for both embedding materials, which is what the image shows.

Blocking

Blocking Details2 hours blocking at room temp using 5% BSA, 1% fish gelatin, 2% donkey serum in PBS

Primary Anitbody

Dilution RatioO/N at 1:1000 dilution in blocking buffer. Also tried in 1:5000 at 4C

Secondary Antibody

Secondary DescriptionAlexaFluor 555 donkey anti-rabbit secondary antibody
Secondary Concentration1 hour in Secondary (1:1000) at room temp

Details

Detection NotesSDC scope
Fixation DetailsEyes fixed in 4% paraformaldehyde for ~2hrs., For paraffin: Standard de-paraffinization
Wash Description4x10 minutes in PBS at room temp

Comments

CommentsThe datasheet said that the antibody would work for western blots and IHC using paraffin sections. Our lab uses both paraffin and frozen sections for IHC, so we tested the antibody on both types of sections. We got comparable results using the antibody for both embedding materials, which is what the image shows.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Opsin 1 (Medium Wave) Antibody (NB110-74730) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Opsin 1 (Medium Wave) Products

Bioinformatics Tool for Opsin 1 (Medium Wave) Antibody (NB110-74730)

Discover related pathways, diseases and genes to Opsin 1 (Medium Wave) Antibody (NB110-74730). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Opsin 1 (Medium Wave) Antibody (NB110-74730)

Discover more about diseases related to Opsin 1 (Medium Wave) Antibody (NB110-74730).
 

Pathways for Opsin 1 (Medium Wave) Antibody (NB110-74730)

View related products by pathway.

PTMs for Opsin 1 (Medium Wave) Antibody (NB110-74730)

Learn more about PTMs related to Opsin 1 (Medium Wave) Antibody (NB110-74730).

Blogs on Opsin 1 (Medium Wave)

There are no specific blogs for Opsin 1 (Medium Wave), but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Henri Leinonen
07/18/2017
Application:
Species: Mouse

 
Anonymous
06/21/2016
Application: IHC-P
Species: Mouse

Anonymous
08/14/2012
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol OPN1MW
Entrez
Uniprot