Opsin 1 (Medium Wave) Antibody

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Protection of the retina against light damage in Grk1-/- mice subjected to BLE (12.5 klux for 0.5 hours) 1 week prior to imaging. Drug combination (M+B+T) or vehicle was injected i.p. 0.5 hours prior to induction of light damage (replicates are presented in Table 2). Representative flat-mount retina image of typical M- (green) and S-cone (blue) populations in vehicle-treated mouse retinas 1 week after BLE. Dashed white lines show border between damaged and healthy site in cone population. M+B+T at a dose 10-1-0.5 mg/kg maintained cone population at ~95%, whereas ONL thickness (representative of rod population) with this dose was decreased by ~50%. M+B+T 50-5-2.5 mg/kg dose led to full protection in both rod and cone population. Image collected and cropped by CiteAb from the following publication (//iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.19-26560) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IF analysis of Opsin 1 in paraffin embedded and frozen mouse retina tissues. Image courtesy of product review submitted by Linda Vuong.

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Retinal whole mount of BALB/c mouse stained against anti M-opsin secondary (AF647) antibody and peanut agglutinin (Fluorescein). This image was submitted via customer Review.

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of rat eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of rat eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of rat eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of mouse eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of mouse eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of mouse eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Opsin 1 (Medium Wave) Antibody Summary

• Immunogen: A synthetic peptide from mouse Opsin 1 (Medium Wave) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OPN1MW
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:250-1:500
  • Immunohistochemistry-Frozen 1:250-1:500
  • Immunohistochemistry-Paraffin 1:250-1:500
  • Western Blot 1:250-1:500
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30307502). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29162627).

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33803057).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for Opsin 1 (Medium Wave) Antibody

• GCP
• GOP
• Green cone photoreceptor pigment
• Green-sensitive opsin
• medium-wave-sensitive opsin 1
• OPN1MW
• opsin 1 (cone pigments), medium-wave-sensitive 2

Background

FUNCTION: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in cone photoreceptor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Opsin 1 (Medium Wave) Antibody (NB110-74730)(15)

Publications using NB110-74730

• Su J, She K, Song L et al. In�vivo base editing rescues photoreceptors in a mouse model of retinitis pigmentosa Molecular Therapy - Nucleic Acids 2023-03-01 [PMID: 36910709] (WB, Mouse)
• Chen B, Liou J, Wu J et al. Photoreceptor and vision protective effects of astragaloside IV in mice model with light-evoked retinal damage Biomedicine & Pharmacotherapy 2022-09-01 [PMID: 36076531] (IHC-P, Mouse)
• Wu J, Yang S, Ho Y et al. The Functional Vision Restorative Effect of Crocin via the BDNF-TrkB Pathway: An In Vivo Study Nutrients 2022-04-20 [PMID: 35565684] (IF/IHC, Mouse)
• Choi EH, Suh S, Foik AT et al. In vivo base editing rescues cone photoreceptors in a mouse model of early-onset inherited retinal degeneration Nature communications 2022-04-05 [PMID: 35383196] (IF/IHC, Mouse)
• Miller LR, Tarantini S, NyUl-TOth A et al. Increased Susceptibility to Cerebral Microhemorrhages Is Associated With Imaging Signs of Microvascular Degeneration in the Retina in an Insulin-Like Growth Factor 1 Deficient Mouse Model of Accelerated Aging Frontiers in aging neuroscience 2022-03-09 [PMID: 35356301] (IHC-P, Mouse)
• Chen Y, Huang Y, Wu P et al. The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo Study Nutrients 2021-03-17 [PMID: 33803057] (IHC-P, Mouse)
• Leinonen H, Pham NC, Boyd T et al. Homeostatic plasticity in the retina is associated with maintenance of night vision during retinal degenerative disease Elife 2020-09-22 [PMID: 32960171] (IF/IHC, Mouse)
• Strayve D, Makia MM, Kakakhel M et al. ROM1 contributes to phenotypic heterogeneity in PRPH2-associated retinal disease Hum. Mol. Genet. 2020-07-27 [PMID: 32716032] (ICC/IF)
• Chakraborty D, Strayve DG, Makia MS et al. Novel molecular mechanisms for Prph2-associated pattern dystrophy FASEB J. 2020-01-01 [PMID: 31914632] (WB, ICC/IF, Mouse)
• Conley SM, Stuck MW, Watson JN et al. Prph2 initiates outer segment morphogenesis but maturation requires Prph2/Rom1 oligomerization. Hum. Mol. Genet. 2018-10-10 [PMID: 30307502] (ICC/IF, Mouse)
• Chakraborty D, Conley SM, Zulliger R, Naash MI. The K153Del PRPH2 mutation differentially impacts photoreceptor structure and function Hum. Mol. Genet. 2016-08-15 [PMID: 27365499] (WB, Mouse)
• Liou J, Yang S, Wang P et al. Protective effect of crocin against the declining of high spatial frequency-based visual performance in mice. Journal of Functional Foods 2018-10-01 (IF/IHC, Mouse)
• Pan M, Hu H, Wang R et al. JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production Cells 2019-08-24 [PMID: 31450635] (IHC-P, Mouse)
• Leinonen, H;Choi, EH;Gardella, A;Kefalov, VJ;Palczewski, K; A Mixture of U.S. Food and Drug Administration-Approved Monoaminergic Drugs Protects the Retina From Light Damage in Diverse Models of Night Blindness Invest. Ophthalmol. Vis. Sci. 2019-04-01 [PMID: 30947334] (IHC-P, Mouse)
• Orban T, Leinonen H, Getter T et al. A Combination of G Protein-Coupled Receptor Modulators Protects Photoreceptors from Degeneration. J Pharmacol Exp Ther. 2019-01-31 [PMID: 29162627] (IHC-Fr, Mouse)

Reviews for Opsin 1 (Medium Wave) Antibody (NB110-74730) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer Immunohistochemistry of intact specimen Mouse 07/18/2017

Summary

• Application: Other
• Sample Tested: mouse retina
• Species: Mouse
• Lot: RB0216-251107

Comments

• Comments: Anti-rabbit AlexaFluor 647 nm secondary antibody

reviewed by: Anonymous - IHC-P Mouse 06/21/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse retina embedded in paraffin
• Species: Mouse

reviewed by: Linda Vuong IHC Mouse 08/14/2012

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse retina embedded in paraffin and frozen medium
• Species: Mouse
• Lot: 08062012

FAQs for Opsin 1 (Medium Wave) Antibody (NB110-74730). (Showing 1 - 1 of 1 FAQs).

1. I would to know if the synthetic peptide used as immunogen to generate the anti-opsin1 antibody (NB110-74730) belongs to the N-terminus or C-terminus of opsin1?
   • The antigen for our Opsin 1 (Medium Wave) antibody [NB110-74730] is located between aa region  2-50, so it is N-terminal.

Bioinformatics

• Gene Symbol: OPN1MW
• Entrez:
  • 14539(Mouse)
  • 89810(Rat)
• Uniprot:
  • O35599(Mouse)