PSMA/FOLH1/NAALADase I Antibody (GCP-04)

Images

 
Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - PSMA expression in human prostate cancer cell lines. Image from verified customer review.
Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.
Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - Fragment of human GCPII (aminoacids 44-750) produced in S2 cells on Western blot by GCP-04 monoclonal antibody. Lanes 1, 2, 3 represent 800, 400 and ...read more
Immunohistochemistry: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in human Medulla oblongata by GCP-04 monoclonal antibody. Mag. 40x; positive astrocytes in white matter.
Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in human prostate by GCP-04 monoclonal antibody. Mag. 400x; positive epithelium of the prostate glands.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
GCP-04
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PSMA/FOLH1/NAALADase I Antibody (GCP-04) Summary

Immunogen
Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells (NM_001014986.1).
Specificity
The antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOLH1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-45057 in the following applications:

Publications
Read Publications using NBP1-45057.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for PSMA/FOLH1/NAALADase I Antibody (GCP-04)

  • Cell growth-inhibiting gene 27 protein
  • cell growth-inhibiting protein 27
  • EC 3.4.17.21
  • FGCP
  • folate hydrolase (prostate-specific membrane antigen) 1
  • Folate hydrolase 1
  • FOLH1
  • FOLHNAALADase I
  • Folylpoly-gamma-glutamate carboxypeptidase
  • GCP2
  • GCPII
  • GCPIImGCP
  • glutamate carboxylase II
  • glutamate carboxypeptidase 2
  • Glutamate carboxypeptidase II
  • Membrane glutamate carboxypeptidase
  • mopsm
  • NAALAD1
  • NAALAD1N-acetylated-alpha-linked acidic dipeptidase I
  • NAALADase I
  • NAALAdase
  • N-acetylated alpha-linked acidic dipeptidase 1
  • prostate specific membrane antigen variant F
  • Prostate-specific membrane antigen
  • PSMA
  • PSMAFGCP
  • PSMPteroylpoly-gamma-glutamate carboxypeptidase

Background

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057)(5)

Reviews for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Human and Canine.

Reviews using NBP1-45057:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Human and Canine
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PSMA/FOLH1/NAALADase I NBP1-45057
Enlarge
reviewed by:
Holly Pondenis
WB Human and Canine 12/15/2017
View

Summary

ApplicationWestern Blot
Sample Testedcanine prostate carcinoma,Human prostate cancer cell line (PC3),LNCaP human prostate cancer cell line
SpeciesHuman and Canine
Lot522819

Comments

CommentsReduce denaturing, 4-20% gel
50ug of protein per lane except LNCaP (5ug)
PVDF membrane
Blocking: 5% Milk TBST, 1 hour room temperature
Dilution 1:250, 4 C overnight
Secondary: rabbit anti-mouse HRP
Detection: ECL femto
Western Blot PSMA/FOLH1/NAALADase I NBP1-45057
Enlarge
reviewed by:
Anonymous
WB Human 04/02/2015
View

Summary

ApplicationWestern Blot
Sample Testedprostate cancer cell line
SpeciesHuman

Blocking

Blocking Details1 hour room temperature in 5% milk TBS-T

Primary Anitbody

Dilution Ratio1:10,000 dilution one hour room temperature

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Thermo Fisher 32260
Secondary Concentration1:10,000 dilution

Details

Detection Notes10 seconds dark room exposure using Millipore Luminata Western HRP reagent

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-45057AF405
Alexa Fluor 488 Labeled NBP1-45057AF488
Alexa Fluor 647 Labeled NBP1-45057AF647
Alexa Fluor 700 Labeled NBP1-45057AF700
DyLight 350 Labeled NBP1-45057UV
DyLight 405 Labeled NBP1-45057V
DyLight 488 Labeled NBP1-45057G
DyLight 680 Labeled NBP1-45057FR
DyLight 755 Labeled NBP1-45057IR

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Bioinformatics Tool for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057)

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Diseases for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057)

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PTMs for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Holly Pondenis
12/15/2017
Application: WB
Species: Human and Canine

Anonymous
04/02/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol FOLH1
Entrez
Uniprot