PSMA/FOLH1/NAALADase I Antibody (GCP-04)

Images

 
Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - PSMA expression in human prostate cancer cell lines. Image from verified customer review.
Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.
Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - Fragment of human GCPII (aminoacids 44-750) produced in S2 cells on Western blot by GCP-04 monoclonal antibody. Lanes 1, 2, 3 represent 800, 400 and ...read more
Immunohistochemistry: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in human Medulla oblongata by GCP-04 monoclonal antibody. Mag. 40x; positive astrocytes in white matter.
Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in human prostate by GCP-04 monoclonal antibody. Mag. 400x; positive epithelium of the prostate glands.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
GCP-04
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PSMA/FOLH1/NAALADase I Antibody (GCP-04) Summary

Immunogen
Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells (NM_001014986.1).
Specificity
The antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOLH1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
Application Notes
PSMA/FOLH1/NAALADase I antibody validated for WB from a verified customer review.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-45057 in the following applications:

Publications
Read Publications using
NBP1-45057 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for PSMA/FOLH1/NAALADase I Antibody (GCP-04)

  • Cell growth-inhibiting gene 27 protein
  • cell growth-inhibiting protein 27
  • EC 3.4.17.21
  • FGCP
  • folate hydrolase (prostate-specific membrane antigen) 1
  • Folate hydrolase 1
  • FOLH1
  • FOLHNAALADase I
  • Folylpoly-gamma-glutamate carboxypeptidase
  • GCP2
  • GCPII
  • GCPIImGCP
  • glutamate carboxylase II
  • glutamate carboxypeptidase 2
  • Glutamate carboxypeptidase II
  • Membrane glutamate carboxypeptidase
  • mopsm
  • NAALAD1
  • NAALAD1N-acetylated-alpha-linked acidic dipeptidase I
  • NAALADase I
  • NAALAdase
  • N-acetylated alpha-linked acidic dipeptidase 1
  • prostate specific membrane antigen variant F
  • Prostate-specific membrane antigen
  • PSMA
  • PSMAFGCP
  • PSMPteroylpoly-gamma-glutamate carboxypeptidase

Background

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057)(8)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IHC, IHC-P.


Filter By Application
IHC
(1)
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-45057 Applications Species
Oliveira D, Stegmayr C, Heinzel A et al. High uptake of 68Ga-PSMA and 18F-DCFPyL in the peritumoral area of rat gliomas due to activated astrocytes EJNMMI Res May 25 2020 [PMID: 32451793] (IHC, Rat) IHC Rat
Dowling M, Samuelson J, Fadl-Alla B et al. Overexpression of prostate specific membrane antigen by canine hemangiosarcoma cells provides opportunity for the molecular detection of disease burdens within hemorrhagic body cavity effusions. PLoS ONE Jan 2 2019 [PMID: 30601866] (IHC-P, Human) IHC-P Human
Rovenska M, Hlouchova K, Sacha P et al. Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs. Prostate. 2008 Feb. [PMID: 18076021]
Sacha P, Zamecnik J, Barinka C et al. Expression of glutamate carboxypeptidase II in human brain. Neuroscience. 2007 Feb. [PMID: 17150306]
Barinka C, Mlcochova P, Sacha P et al. Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding. Eur J Biochem. 2004 Jul. [PMID: 15206943]
Barinka C, Sacha P, Sklenar J et al. Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity. Protein Sci. 2004 Jun. [PMID: 15152093]
Barinka C, Rinnova M, Sacha P et al. Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II. J Neurochem. 2002 Feb. [PMID: 11905994]
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018

Reviews for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Human and Canine.

Reviews using NBP1-45057:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Human and Canine
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PSMA/FOLH1/NAALADase I NBP1-45057
Enlarge
reviewed by:
Holly Pondenis
WB Human and Canine 12/15/2017
View

Summary

ApplicationWestern Blot
Sample Testedcanine prostate carcinoma,Human prostate cancer cell line (PC3),LNCaP human prostate cancer cell line
SpeciesHuman and Canine
Lot522819

Comments

CommentsReduce denaturing, 4-20% gel
50ug of protein per lane except LNCaP (5ug)
PVDF membrane
Blocking: 5% Milk TBST, 1 hour room temperature
Dilution 1:250, 4 C overnight
Secondary: rabbit anti-mouse HRP
Detection: ECL femto
Western Blot PSMA/FOLH1/NAALADase I NBP1-45057
Enlarge
reviewed by:
Anonymous
WB Human 04/02/2015
View

Summary

ApplicationWestern Blot
Sample Testedprostate cancer cell line
SpeciesHuman

Blocking

Blocking Details1 hour room temperature in 5% milk TBS-T

Primary Anitbody

Dilution Ratio1:10,000 dilution one hour room temperature

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Thermo Fisher 32260
Secondary Concentration1:10,000 dilution

Details

Detection Notes10 seconds dark room exposure using Millipore Luminata Western HRP reagent

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-45057AF350
Alexa Fluor 405 NBP1-45057AF405
Alexa Fluor 488 NBP1-45057AF488
Alexa Fluor 532 NBP1-45057AF532
Alexa Fluor 594 NBP1-45057AF594
Alexa Fluor 647 NBP1-45057AF647
Alexa Fluor 700 NBP1-45057AF700
Alexa Fluor 750 NBP1-45057AF750
DyLight 350 NBP1-45057UV
DyLight 405 NBP1-45057V
DyLight 488 NBP1-45057G
DyLight 550 NBP1-45057R
DyLight 594 NBP1-45057DL594
DyLight 650 NBP1-45057C
DyLight 680 NBP1-45057FR
DyLight 755 NBP1-45057IR
Janelia Fluor 549 NBP1-45057JF549
Janelia Fluor 646 NBP1-45057JF646

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Holly Pondenis
12/15/2017
Application: WB
Species: Human and Canine

Anonymous
04/02/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol FOLH1
Entrez
Uniprot