PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free

Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - Analysis of human PSMA using mouse monoclonal antibody GCP-04 on lysates of LNCaP cell line and Jurkat cell line (PSMA non-expressing cell line; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of mouse anti-PSMA monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody.

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.

Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - PSMA expression in human prostate cancer cell lines. Image from verified customer review.

Immunohistochemistry: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in human Medulla oblongata by GCP-04 monoclonal antibody. Mag. 40x; positive astrocytes in white matter.

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in human prostate by GCP-04 monoclonal antibody. Mag. 400x; positive epithelium of the prostate glands.

Immunohistochemistry: PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free [NBP1-45057] - Ga-PSMA PET and PSMA/FOLH1/NAALADase I immunohistochemistry of representative GBM. A) MRI, 68Ga-PSMA PET, and fused images. B) PSMA/FOLH1/NAALADase I expression in GBM vascular endothelium. C) No PSMA/FOLH1/NAALADase I expression in normal brain. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35517711/) licensed under a CC-BY license.

68Ga-PSMA PET and PSMA immunohistochemistry of representative GBM. (a) MRI, 68Ga-PSMA PET, and fused images. (b) PSMA expression in GBM vascular endothelium. (c) No PSMA expression in normal brain. Reproduced with permission from Schwenck et al. [17].

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ICC/IF, IHC
• Clone: GCP-04
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free Summary

• Immunogen: Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells (NM_001014986.1).
• Specificity: The antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOLH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1 ug/ml
• Application Notes: PSMA/FOLH1/NAALADase I antibody validated for WB from a verified customer review. Western blotting: Recommended dilution: 1 ug/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free

• Cell growth-inhibiting gene 27 protein
• cell growth-inhibiting protein 27
• EC 3.4.17.21
• FGCP
• folate hydrolase (prostate-specific membrane antigen) 1
• Folate hydrolase 1
• FOLH1
• FOLHNAALADase I
• Folylpoly-gamma-glutamate carboxypeptidase
• GCP2
• GCPII
• GCPIImGCP
• glutamate carboxylase II
• glutamate carboxypeptidase 2
• Glutamate carboxypeptidase II
• Membrane glutamate carboxypeptidase
• mopsm
• NAALAD1
• NAALAD1N-acetylated-alpha-linked acidic dipeptidase I
• NAALADase I
• NAALAdase
• N-acetylated alpha-linked acidic dipeptidase 1
• prostate specific membrane antigen variant F
• Prostate-specific membrane antigen
• PSMA
• PSMAFGCP
• PSMPteroylpoly-gamma-glutamate carboxypeptidase

Background

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057)(10)

Publications using NBP1-45057

• Berry MR, Fadl-Alla BA, Samuelson J et al. Investigating PSMA differential expression in canine uroepithelial carcinomas to aid disease-based stratification and guide therapeutic selection BMC veterinary research 2022-12-20 [PMID: 36539731] (IHC, WB, ICC/IF, Canine, Human)
• Stopa B, Crowley J, JuhAsz C et al. Prostate-Specific Membrane Antigen as Target for Neuroimaging of Central Nervous System Tumors Molecular Imaging 2022-04-15 [PMID: 35517711]
• Dowling M, Samuelson J, Fadl-Alla B et al. Overexpression of prostate specific membrane antigen by canine hemangiosarcoma cells provides opportunity for the molecular detection of disease burdens within hemorrhagic body cavity effusions. PLoS ONE 2019-01-02 [PMID: 30601866] (IHC-P, Human)
• Rovenska M, Hlouchova K, Sacha P et al. Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs. Prostate. 2008-02-01 [PMID: 18076021]
• Sacha P, Zamecnik J, Barinka C et al. Expression of glutamate carboxypeptidase II in human brain. Neuroscience. 2007-02-01 [PMID: 17150306]
• Barinka C, Mlcochova P, Sacha P et al. Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding. Eur J Biochem. 2004-07-01 [PMID: 15206943]
• Barinka C, Sacha P, Sklenar J et al. Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity. Protein Sci. 2004-06-01 [PMID: 15152093]
• Barinka C, Rinnova M, Sacha P et al. Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II. J Neurochem. 2002-02-01 [PMID: 11905994]
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
• Oliveira D, Stegmayr C, Heinzel A et al. High uptake of 68Ga-PSMA and 18F-DCFPyL in the peritumoral area of rat gliomas due to activated astrocytes EJNMMI Res 2020-05-25 [PMID: 32451793] (IF/IHC, Rat)

Reviews for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Canine and Human.

reviewed by: Holly Pondenis WB Canine and Human 12/15/2017

Summary

• Application: Western Blot
• Sample Tested: canine prostate carcinoma,Human prostate cancer cell line (PC3),LNCaP human prostate cancer cell line
• Species: Canine and Human
• Lot: 522819

Comments

• Comments: Reduce denaturing, 4-20% gel
  50ug of protein per lane except LNCaP (5ug)
  PVDF membrane
  Blocking: 5% Milk TBST, 1 hour room temperature
  Dilution 1:250, 4 C overnight
  Secondary: rabbit anti-mouse HRP
  Detection: ECL femto

reviewed by: Verified Customer WB Human 04/02/2015

Summary

• Application: Western Blot
• Sample Tested: prostate cancer cell line
• Species: Human

Bioinformatics

• Gene Symbol: FOLH1
• Entrez:
  • 2346(Human)
  • 397677(Rat)
  • 85309(Rat)
• Uniprot:
  • Q04609()