PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free

Images

 
Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - Analysis of human PSMA using mouse monoclonal antibody GCP-04 on lysates of LNCaP cell line and Jurkat cell line (PSMA non-expressing cell line; ...read more
Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.
Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - PSMA expression in human prostate cancer cell lines. Image from verified customer review.
Immunohistochemistry: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in human Medulla oblongata by GCP-04 monoclonal antibody. Mag. 40x; positive astrocytes in white matter.
Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in human prostate by GCP-04 monoclonal antibody. Mag. 400x; positive epithelium of the prostate glands.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
GCP-04
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free Summary

Immunogen
Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells (NM_001014986.1).
Specificity
The antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOLH1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1 ug/ml
Application Notes
PSMA/FOLH1/NAALADase I antibody validated for WB from a verified customer review. Western blotting: Recommended dilution: 1 ug/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-45057 in the following applications:

Publications
Read Publications using
NBP1-45057 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free

  • Cell growth-inhibiting gene 27 protein
  • cell growth-inhibiting protein 27
  • EC 3.4.17.21
  • FGCP
  • folate hydrolase (prostate-specific membrane antigen) 1
  • Folate hydrolase 1
  • FOLH1
  • FOLHNAALADase I
  • Folylpoly-gamma-glutamate carboxypeptidase
  • GCP2
  • GCPII
  • GCPIImGCP
  • glutamate carboxylase II
  • glutamate carboxypeptidase 2
  • Glutamate carboxypeptidase II
  • Membrane glutamate carboxypeptidase
  • mopsm
  • NAALAD1
  • NAALAD1N-acetylated-alpha-linked acidic dipeptidase I
  • NAALADase I
  • NAALAdase
  • N-acetylated alpha-linked acidic dipeptidase 1
  • prostate specific membrane antigen variant F
  • Prostate-specific membrane antigen
  • PSMA
  • PSMAFGCP
  • PSMPteroylpoly-gamma-glutamate carboxypeptidase

Background

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057)(9)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IHC, IHC-P.


Filter By Application
IHC
(1)
IHC-P
(1)
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Filter By Species
Human
(1)
Rat
(1)
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Showing Publications 1 - 9 of 9.
Publications using NBP1-45057 Applications Species
Stopa B, Crowley J, JuhAsz C et al. Prostate-Specific Membrane Antigen as Target for Neuroimaging of Central Nervous System Tumors Molecular Imaging Apr 15 2022 [PMID: 35517711]
Dowling M, Samuelson J, Fadl-Alla B et al. Overexpression of prostate specific membrane antigen by canine hemangiosarcoma cells provides opportunity for the molecular detection of disease burdens within hemorrhagic body cavity effusions. PLoS ONE Jan 2 2019 [PMID: 30601866] (IHC-P, Human) IHC-P Human
Rovenska M, Hlouchova K, Sacha P et al. Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs. Prostate. 2008 Feb. [PMID: 18076021]
Sacha P, Zamecnik J, Barinka C et al. Expression of glutamate carboxypeptidase II in human brain. Neuroscience. 2007 Feb. [PMID: 17150306]
Barinka C, Mlcochova P, Sacha P et al. Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding. Eur J Biochem. 2004 Jul. [PMID: 15206943]
Barinka C, Sacha P, Sklenar J et al. Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity. Protein Sci. 2004 Jun. [PMID: 15152093]
Barinka C, Rinnova M, Sacha P et al. Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II. J Neurochem. 2002 Feb. [PMID: 11905994]
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018
Oliveira D, Stegmayr C, Heinzel A et al. High uptake of 68Ga-PSMA and 18F-DCFPyL in the peritumoral area of rat gliomas due to activated astrocytes EJNMMI Res May 25 2020 [PMID: 32451793] (IHC, Rat) IHC Rat

Reviews for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Canine and Human.

Reviews using NBP1-45057:
Filter by Applications
Western Blot
(2)
All Applications
Filter by Species
Human
(1)
Canine and Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PSMA/FOLH1/NAALADase I NBP1-45057
Enlarge
4
reviewed by:
Verified Customer
Western Blot Canine and Human 12/15/2017
View

Summary

ApplicationWestern Blot
Sample Testedcanine prostate carcinoma,Human prostate cancer cell line (PC3),LNCaP human prostate cancer cell line
SpeciesCanine and Human
Lot522819

Comments

CommentsReduce denaturing, 4-20% gel
50ug of protein per lane except LNCaP (5ug)
PVDF membrane
Blocking: 5% Milk TBST, 1 hour room temperature
Dilution 1:250, 4 C overnight
Secondary: rabbit anti-mouse HRP
Detection: ECL femto
Western Blot PSMA/FOLH1/NAALADase I NBP1-45057
Enlarge
5
reviewed by:
Clarisse Marotz
Western Blot Human 04/02/2015
View

Summary

ApplicationWestern Blot
Sample Testedprostate cancer cell line
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSMA/FOLH1/NAALADase I Antibody (NBP1-45057) (0)

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Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
12/15/2017
Application: Western Blot
Species: Canine and Human

Clarisse Marotz
04/02/2015
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol FOLH1
Entrez
Uniprot