Human CXCL6/GCP-2 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CXCL6/GCP-2 Quantikine ELISA Kit Summary

• Background: The Quantikine Human GCP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human GCP-2 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human GCP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human GCP-2 showed linear curves that were parallel to the standard curves obtaine...d using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GCP-2.
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• Specificity: Natural and recombinant human GCP-2
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CXCL6

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CXCL6/GCP-2 Quantikine ELISA Kit

• chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2)
• Chemokine alpha 3
• CKA-3Granulocyte chemotactic protein 2
• CXCL6
• GCP-2
• GCP2member 6 (granulocytechemotactic protein 2)
• GCP-2Small-inducible cytokine B6
• Small inducible cytokine subfamily B (Cys-X-Cys), member b

Background

GCP-2 (granulocyte chemotactic protein-2) is a CXC chemokine. Among human CXC chemokines, GCP-2 is most closely related to ENA-78. The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity, suggesting the two genes may have originated from a recent gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The mouse protein designated GCP-2, because of its amino acid sequence similarity (60%) to human GCP-2, is identical to the LIX protein sequence.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CXCL6/GCP-2 (DGC00)(8)

Publications using DGC00

• MJ Sebastião, M Serra, R Pereira, I Palacios, P Gomes-Alve, PM Alves Human cardiac progenitor cell activation and regeneration mechanisms: exploring a novel myocardial ischemia/reperfusion in vitro model Stem Cell Res Ther, 2019-03-07;10(1):77. 2019-03-07 [PMID: 30845956] (Human)
• SL Fossum, MJ Mutolo, A Tugores, S Ghosh, SH Randell, LC Jones, SH Leir, A Harris Ets Homologous Factor (EHF) has Critical Roles in Epithelial Dysfunction in Airway Disease J. Biol. Chem., 2017-05-01;0(0):. 2017-05-01 [PMID: 28461336] (Human)
• Rudisch A, Dewhurst M, Horga L, Kramer N, Harrer N, Dong M, van der Kuip H, Wernitznig A, Bernthaler A, Dolznig H, Sommergruber W High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts. PLoS ONE, 2015-04-28;10(4):e0124283. 2015-04-28 [PMID: 25919140] (Human)
• Matsuda A, Fukuda S, Matsumoto K, Saito H Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis. Am. J. Respir. Cell Mol. Biol., 2007-08-20;38(2):168-75. 2007-08-20 [PMID: 17709600] (Human)
• Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion. Atherosclerosis, 2006-10-04;192(1):56-66. 2006-10-04 [PMID: 17022986] (Human)
• Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J. Immunol., 2005-11-01;175(9):6177-89. 2005-11-01 [PMID: 16237115] (Human)
• Gessler P, Pretre R, Hohl V, Rousson V, Fischer J, Dahinden C CXC-chemokine stimulation of neutrophils correlates with plasma levels of myeloperoxidase and lactoferrin and contributes to clinical outcome after pediatric cardiac surgery. Shock, 2004-12-01;22(6):513-20. 2004-12-01 [PMID: 15545821] (Human)
• Maheshwari A, Christensen RD, Calhoun DA ELR+ CXC chemokines in human milk. Cytokine, 2003-11-07;24(3):91-102. 2003-11-07 [PMID: 14581003] (Human)

FAQs for CXCL6/GCP-2 (DGC00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CXCL6