OBFC2B Antibody (OTI1E11) - Azide and BSA Free

Western Blot: OBFC2B Antibody (OTI1E11) - Azide and BSA Free [NBP2-73140] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OBFC2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OBFC2B.

Immunohistochemistry: OBFC2B Antibody (OTI1E11) - Azide and BSA Free [NBP2-73140] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OBFC2B mouse monoclonal antibody.

Flow Cytometry: OBFC2B Antibody (OTI1E11) - Azide and BSA Free [NBP2-73140] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-OBFC2B antibody, and then analyzed by flow cytometry.

Western Blot: OBFC2B Antibody (OTI1E11) - Azide and BSA Free [NBP2-73140] - Analysis of extracts (35ug) from 9 different cell lines by using anti-OBFC2B monoclonal antibody.

Immunohistochemistry: OBFC2B Antibody (OTI1E11) - Azide and BSA Free [NBP2-73140] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-OBFC2B mouse monoclonal antibody.

Immunohistochemistry: OBFC2B Antibody (OTI1E11) - Azide and BSA Free [NBP2-73140] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OBFC2B mouse monoclonal antibody.

Immunohistochemistry: OBFC2B Antibody (OTI1E11) - Azide and BSA Free [NBP2-73140] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-OBFC2B mouse monoclonal antibody.

Immunohistochemistry: OBFC2B Antibody (OTI1E11) - Azide and BSA Free [NBP2-73140] - Staining of paraffin-embedded Human breast tissue using anti-OBFC2B mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI1E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

OBFC2B Antibody (OTI1E11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse OBFC2B Antibody (OTI1E11) - Azide and BSA Free (NBP2-01578) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human OBFC2B(NP_076973) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NABP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 22.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for OBFC2B Antibody (OTI1E11) - Azide and BSA Free

• LP3587
• MGC2731
• oligonucleotide/oligosaccharide-binding fold containing 2B
• Oligonucleotide/oligosaccharide-binding fold-containing protein 2B
• Sensor of single-strand DNA complex subunit B1
• Sensor of ssDNA subunit B1
• Single-stranded DNA-binding protein 1
• SOSS complex subunit B1
• SOSS-B1
• SSB1hSSB1

Background

Single-stranded DNA -binding protein (SSB1) is phosphorylated by ATM in response to DNA damage. SSB1 is required for checkpoint activation and is critical for genomic stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NABP2