O-GlcNAcase/OGA/MGEA5 Antibody - BSA Free

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Section of human testicular seminoma. Antibody: Affinity purified rabbit anti- MGEA5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Immunoprecipitation: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MGEA5 antibody NBP2-32233 used for IP at 6 ug per reaction. MGEA5 was also immunoprecipitated by rabbit anti-MGEA5 antibody BL15772. For blotting immunoprecipitated MGEA5, NBP2-32233 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot- O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Human MGEA5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MGEA5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

O-GlcNAcase/OGA/MGEA5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit O-GlcNAcase/OGA/MGEA5 Antibody - BSA Free (NBP2-32233) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-O-GlcNAcase/OGA/MGEA5 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 500 to 550 of human Meningioma Expressed Antigen 5 using the numbering given in entry NP_036347.1 (GeneID 10724).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OGA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for O-GlcNAcase/OGA/MGEA5 Antibody - BSA Free

• bifunctional protein NCOAT
• FLJ11229
• GH84
• HEXC
• HEXC3
• Hexosaminidase B
• hyaluronidase in meningioma
• KIAA0679
• MEA5FLJ23355
• meningioma expressed antigen 5 (hyaluronidase)
• Meningioma-expressed antigen 5
• MGEA5
• NCOAT
• Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
• OGA
• OGlcNAcase
• O-GlcNAcase

Background

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for O-GlcNAcase/OGA/MGEA5 Antibody (NBP2-32233)(2)

Publications using NBP2-32233

• Pagesy P, Bouaboud A, Feng Z et al. Short O-GlcNAcase Is Targeted to the Mitochondria and Regulates Mitochondrial Reactive Oxygen Species Level Cells 2022-06-02 [PMID: 35681522] (WB, Mouse, Human)
• Al-Mukh, H, Baudoin, L Et al. Lipopolysaccharide Induces GFAT2 Expression to Promote O-Linked beta -N-Acetylglucosaminylation and Attenuate Inflammation in Macrophages. J Immunol 2020-11-01 [PMID: 32978282] (Chemotaxis, Human)

Bioinformatics

• Gene Symbol: OGA
• Uniprot:
  • NP_036347.1(Human)