O-GlcNAcase/OGA/MGEA5 Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 488

O-GlcNAcase/OGA/MGEA5 Antibody [Alexa Fluor™ Plus 488] Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 500 to 550 of human Meningioma Expressed Antigen 5 using the numbering given in entry NP_036347.1 (GeneID 10724).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OGA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for O-GlcNAcase/OGA/MGEA5 Antibody [Alexa Fluor™ Plus 488]

• bifunctional protein NCOAT
• FLJ11229
• GH84
• HEXC
• HEXC3
• Hexosaminidase B
• hyaluronidase in meningioma
• KIAA0679
• MEA5FLJ23355
• meningioma expressed antigen 5 (hyaluronidase)
• Meningioma-expressed antigen 5
• MGEA5
• NCOAT
• Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
• OGA
• OGlcNAcase
• O-GlcNAcase

Background

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OGA