O-GlcNAc Transferase p110 subunit Antibody

Images

 
Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse tissue extracts (50 ug) was separated by 7.5 % SDS-PAGE, and blotted with O-GlcNAc transferase antibody diluted by 1:2000
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O- GlcNAc ...read more
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Paraffin-embedded rat hind brain. O-GlcNAc transferase antibody diluted at 1:500.
Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - A. 30 ug PC-12 whole cell extract 5 % SDS-PAGE O-GlcNAc transferase antibody dilution: 1:1000
Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract5 % SDS-PAGE ...read more
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc ...read more
Immunoprecipitation: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 ug of O-GlcNAc transferase antibody. Western blot ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

O-GlcNAc Transferase p110 subunit Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.
Localization
Cytoplasm (Potential), Nucleus
Predicted Species
Porcine (100%), Bovine (100%), Xenopus (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OGT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-32791.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for O-GlcNAc Transferase p110 subunit Antibody

  • EC 2.4.1
  • EC 2.4.1.255
  • FLJ23071
  • HRNT1
  • MGC22921
  • O-GlcNAc transferase p110 subunit
  • O-GlcNAc transferase subunit p110
  • OGlcNAc Transferase
  • O-GlcNAc Transferase
  • O-GLCNAC
  • OGT
  • O-linked N-acetylglucosamine (GlcNAc) transferase(UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
  • O-linked N-acetylglucosamine transferase 110 kDa subunit
  • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDasubunit
  • uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase

Background

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for O-GlcNAc Transferase p110 subunit Antibody (NBP1-32791)(1)

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol OGT
Entrez