O-GlcNAc Transferase p110 subunit Antibody

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Scale bar= 10um.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Rat brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - A. 30 ug PC-12 whole cell extract 5 % SDS-PAGE O-GlcNAc transferase antibody dilution: 1:1000

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse tissue extracts (50 ug) was separated by 7.5 % SDS-PAGE, and blotted with O-GlcNAc transferase antibody diluted by 1:2000

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody ) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Rat hind brain. O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse brain.O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Rat brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 ug of O-GlcNAc transferase antibody. Western blot analysis was performed using O-GlcNAc transferase antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Po, Bv, Xp
• Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

O-GlcNAc Transferase p110 subunit Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.
• Localization: Cytoplasm (Potential), Nucleus
• Predicted Species: Porcine (100%), Bovine (100%), Xenopus (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OGT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Application Notes: Flow- Assay dependent.
• Theoretical MW: 117 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for O-GlcNAc Transferase p110 subunit Antibody

• EC 2.4.1
• EC 2.4.1.255
• FLJ23071
• HRNT1
• MGC22921
• O-GlcNAc transferase p110 subunit
• O-GlcNAc transferase subunit p110
• OGlcNAc Transferase
• O-GlcNAc Transferase
• O-GLCNAC
• OGT
• O-linked N-acetylglucosamine (GlcNAc) transferase(UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• O-linked N-acetylglucosamine transferase 110 kDa subunit
• UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDasubunit
• uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase

Background

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for O-GlcNAc Transferase p110 subunit Antibody (NBP1-32791)(4)

Publications using NBP1-32791

• Shen H, Zhao X, Chen J Et al. O-GlcNAc transferase Ogt regulates embryonic neuronal development through modulating Wnt/ Beta -catenin signaling Human molecular genetics 2021-08-04 [PMID: 34346496]
• Cheng J, Wu Y, Chen L et al. Loss of O-GlcNAc transferase in neural stem cells impairs corticogenesis Biochem. Biophys. Res. Commun. 2020-09-04 [PMID: 32896380] (WB, Mouse)
• White CW, Fan X, Maynard JC et al. Age-related loss of neural stem cell O-GlcNAc promotes a glial fate switch through STAT3 activation Proc. Natl. Acad. Sci. U.S.A. 2020-08-26 [PMID: 32848054] (WB, Mouse)
• Ise H, Goto M, Komura K et al. Engulfment and clearance of apoptotic cells based on a GlcNAc-binding lectin-like property of surface vimentin. Glycobiology 2012-06-01 [PMID: 22345628] (WB)

Bioinformatics

• Gene Symbol: OGT