Numb Antibody

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Western Blot: NUMB Antibody [NBP2-27181] - Analysis of mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NUMB antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody (20301) and ...read more
Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human adrenal tissue stained with NUMB antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human spleen stained with NUMB antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Pm, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Numb Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 12-40 of human NUMB was used as the immunogen for the antibody, GenBank no gb|AAH20788.1|.
Predicted Species
Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUMB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10ug/ml~
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in Immunohistochemistry reported in scientific literature (PMID: 30256757).
Publications
Read Publication using
NBP2-27181 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Numb Antibody

  • h-Numb
  • mnb
  • m-numb
  • numb (Drosophila) homolog
  • numb homolog (Drosophila)
  • Numb
  • protein numb homolog
  • Protein S171
  • S171

Background

Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Numb Antibody (NBP2-27181)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Rat
(1)
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Bioinformatics

Gene Symbol NUMB
Uniprot