NPM1 Overexpression Lysate

Images

 
Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.
Western Blot: Nucleophosmin Overexpression Lysate (Adult Normal) [NBL1-13748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleophosmin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NPM1 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203841 and based on accession number NM_199185. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens nucleophosmin (nucleolar phosphoprotein B23, numatrin) (NPM1), transcript variant 2, mRNA.
Gene
NPM1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
29.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBL1-13748 in the following application:

Publications
Read Publication using NBL1-13748.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NPM1 Overexpression Lysate

  • B23
  • MGC104254
  • NPM1
  • NPMnucleophosmin/nucleoplasmin family, member 1
  • Nucleolar phosphoprotein B23
  • Nucleolar protein NO38
  • nucleophosmin (nucleolar phosphoprotein B23, numatrin)
  • Nucleophosmin
  • Numatrin

Background

NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM]. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2) that lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for NPM1 Lysate (NBL1-13748)(1)

We have publications tested in 1 application: WB.


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Review for NPM1 Lysate (NBL1-13748) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBL1-13748:
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Images Ratings Applications Species Date Details
Western Blot NPM1 NBL1-13748
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reviewed by:
Tereza Toralova
WB 10/12/2012
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Summary

ApplicationWestern Blot
Lot0512
Special ApplicationsExcellent for testing antibody specifity

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NBL1-13748

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Recent Reviews

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Tereza Toralova
10/12/2012
Application: WB
Species:

Bioinformatics

Gene Symbol NPM1