NPM1 Antibody [DyLight 488]

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 488

NPM1 Antibody [DyLight 488] Summary

• Immunogen: A synthetic peptide made to a C-terminal region of human mutant Nucleophosmin (within residues 250-294). [Accession# AAW67752]
• Localization: Nucleophosmin typically resides in the nucleus. However, in patients with acute myelogenous leukemia (AML) it mutates and localizes to the cytoplasm.
• Specificity: This antibody is specific for the mutant form of Nucleophosmin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30214626).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for NPM1 Antibody [DyLight 488]

• B23
• MGC104254
• NPM1
• NPMnucleophosmin/nucleoplasmin family, member 1
• Nucleolar phosphoprotein B23
• Nucleolar protein NO38
• nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Nucleophosmin
• Numatrin

Background

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPM1 Antibody (NB110-61646G)(1)

Publication using NB110-61646G

• El-Gamal RAE, Hashem AE, Habashy DM et al. Flow cytometry in detection of Nucleophosmin 1 mutation in acute myeloid leukemia patients: A reproducible tertiary hospital experience Int J Lab Hematol 2020-08-27 [PMID: 32856429]
  
  Details:
  Citation using the DyLight 488 format of this antibody.

FAQs for NPM1 Antibody (NB110-61646G). (Showing 1 - 1 of 1 FAQ).

1. Does the rabbit polyclonal anti-Nucleophosmin Antibody [DyLight 488] (NB110-61646G) react with the exon 12 mutant NPM1?
   • From the data I am showing this antibody should detect mutant NPM1 from our testing.

Bioinformatics

• Gene Symbol: NPM1