p23/PTGES3 Antibody (JJ3)

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Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 25 ug of Hela (Lane 1), HepG2 (Lane 2), and mouse spleen cell lysates (Lane 3) and a molecular weight protein ladder.
Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and ...read more
Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Normal biopsies of deparaffinized Human testis tissue.
Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 3T3 cells compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and ...read more
Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and ...read more
Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.
Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Normal biopsies of deparaffinized Human tonsil tissue.
Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of Jurkat cells compared to an isotype control (blue).
Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of Hela cells compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, GP, Pm, Rb, XpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
JJ3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

p23/PTGES3 Antibody (JJ3) Summary

Immunogen
Recombinant human p23 protein.
Specificity
p23 (JJ3)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTGES3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 23 kDa protein representing p23 from rabbit reticulocyte lysate. May be useful in IP for both free and complexed p23.
Positive Control
p23/PTGES3 Lysate (NBL1-14937)
p23/PTGES3 Lysate (NBP2-66043)
Publications
Read Publications using
NB300-576 in the following applications:

  • WB
    4 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 16085934). Primate reactivity reported in scientific literature (PMID: 11553639). Clawed Frog reactivity reported in scientific literature (PMID: 18364744). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for p23/PTGES3 Antibody (JJ3)

  • CPGES
  • cPGESp23
  • Cytosolic prostaglandin E2 synthase
  • Hsp90 co-chaperone
  • p23
  • P23cytosolic prostaglandin E synthase
  • Progesterone receptor complex p23
  • prostaglandin E synthase 3 (cytosolic)
  • prostaglandin E synthase 3
  • PTGES3
  • TEBP
  • TEBPEC 5.3.99.3
  • Telomerase-binding protein p23
  • unactive progesterone receptor, 23 kD

Background

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP 70 and HSP 90), immunosuppressant binding proteins called immunophilins (the FK 506 binding proteins, FKBP 52 & FKBP 54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP 70, HSP 90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP 90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for p23/PTGES3 Antibody (NB300-576)(4)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PTGES3
Entrez