p23/PTGES3 Antibody (JJ3)

Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 25 ug of Hela (Lane 1), HepG2 (Lane 2), and mouse spleen cell lysates (Lane 3) and a molecular weight protein ladder.

Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Normal biopsies of deparaffinized Human testis tissue.

Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 3T3 cells compared to an isotype control (blue).

Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Normal biopsies of deparaffinized Human tonsil tissue.

Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of Jurkat cells compared to an isotype control (blue).

Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of Hela cells compared to an isotype control (blue).

• Reactivity: Hu, Mu, Rt, Ch, Gp, Pm, Rb, Xp
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: JJ3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

p23/PTGES3 Antibody (JJ3) Summary

• Immunogen: Recombinant human p23 protein.
• Specificity: p23 (JJ3)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTGES3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 23 kDa protein representing p23 from rabbit reticulocyte lysate. May be useful in IP for both free and complexed p23.
• Control:

  p23/PTGES3 Overexpression Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 16085934). Primate reactivity reported in scientific literature (PMID: 11553639). Clawed Frog reactivity reported in scientific literature (PMID: 18364744). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for p23/PTGES3 Antibody (JJ3)

• CPGES
• cPGESp23
• Cytosolic prostaglandin E2 synthase
• Hsp90 co-chaperone
• p23
• P23cytosolic prostaglandin E synthase
• Progesterone receptor complex p23
• prostaglandin E synthase 3 (cytosolic)
• prostaglandin E synthase 3
• PTGES3
• TEBP
• TEBPEC 5.3.99.3
• Telomerase-binding protein p23
• unactive progesterone receptor, 23 kD

Background

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP 70 and HSP 90), immunosuppressant binding proteins called immunophilins (the FK 506 binding proteins, FKBP 52 & FKBP 54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP 70, HSP 90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP 90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p23/PTGES3 Antibody (NB300-576)(8)

Publications using NB300-576

• He X, Smith SE, Chen S et al. Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche Cell reports 2021-09-07 [PMID: 34496236]
• de Vries S, Benes V, Naarmann-de Vries IS et al. P23 Acts as Functional RBP in the Macrophage Inflammation Response Frontiers in molecular biosciences 2021-06-11 [PMID: 34179071] (IP, Mouse)
• Vilas A, Yuste-Checa P, Gallego D et Al. Proteostasis regulators as potential rescuers of PMM2 activity Biochim Biophys Acta Mol Basis Dis 2020-03-25 [PMID: 32222543] (WB, Human)
• Lackie RE, Razzaq AR, Farhan SMK et al. Modulation of hippocampal neuronal resilience during aging by the Hsp70/Hsp90 co-chaperone STI1 J. Neurochem. 2019-09-28 [PMID: 31562773] (WB, Mouse)
• Garcia-Belinchon M, Sanchez-Osuna M, Martinez-Escardo L et al. An Early and Robust Activation of Caspases Heads Cells for a Regulated Form of Necrotic-like Cell Death J. Biol. Chem. 2015-06-29 [PMID: 26124276] (WB, Human)
• Sanchez-Osuna M, Garcia-Belinchon M, Iglesias-Guimarais V et al. Caspase-Activated DNase is necessary and sufficient for oligonucleosomal DNA breakdown, but not for chromatin disassembly during caspase-dependent apoptosis of LN-18 glioblastoma cells. J. Biol. Chem. 2014-05-17 [PMID: 24838313] (WB, Human)
• Casanelles E, Gozzelino R, Marques-Fernandez F et al. NF-kappaB activation fails to protect cells to TNFalpha-induced apoptosis in the absence of Bcl-xL, but not Mcl-1, Bcl-2 or Bcl-w. Biochim Biophys Acta 2013-01-28 [PMID: 23369735] (WB, Human)
• Iglesias-Guimarais V, Gil-Guinon E, Gabernet G et al. Apoptotic DNA Degradation into Oligonucleosomal Fragments, but Not Apoptotic Nuclear Morphology, Relies on a Cytosolic Pool of DFF40/CAD Endonuclease. J Biol Chem 2012-01-17 [PMID: 22253444] (WB, Human)

Bioinformatics

• Gene Symbol: PTGES3
• Entrez:
  • 10728(Human)