Nucleolin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody [NB600-241] - Analysis of Nucleolin MCF-7 breast cancer cells. Image courtsey of product review by Lacey Litchfield.
Simple Western: Nucleolin Antibody [NB600-241] - Image shows a specific band for Nucleolin in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody [NB600-241] - Endogenous nuclear nucleolin-COUP-TFII interaction in MCF-7 and T47D cells.NE (200A ug protein) from MCF-7 cells. Immunofluorescent staining of ...read more
Western Blot: Nucleolin Antibody [NB600-241] - Detection of nucleolin in crude PD31 nuclear extracts.
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). ...read more
Immunohistochemistry: Nucleolin Antibody [NB600-241] - Detection of Nucleolin in human tonsil germinal center.
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody [NB600-241] - In-situ immunofluorescent staining of PD31 murine pre-B cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, PlSpecies Glossary
Applications WB, Simple Western, ChIP, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IHC-WhMt, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Nucleolin Antibody Summary

Immunogen
A fusion protein containing amino acids 284-709 of human Nucleolin. [UniProt# P19338]
Localization
Nucleoli
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCL
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:4000
  • Simple Western 1:400
  • Chromatin Immunoprecipitation
  • Electron Microscopy 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunomicroscopy
  • Immunohistochemistry Whole-Mount
  • Knockdown Validated
Application Notes
Chromatin Immunoprecipitation was reported in scientific literature. In Western blot, a band at 1~106 kDa represents human Nucleolin. Heat mediated antigen retrieval is recommended for IHC-P.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30411850). Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 22483616).
Theoretical MW
106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Nucleolin Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB600-241 in the following applications:

Publications
Read Publications using
NB600-241 in the following applications:

  • 4 publications
  • 6 publications
  • IHC
    1 publication
  • 1 publication
  • IM
    1 publication
  • WB
    8 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Nucleolin Antibody

  • C23
  • FLJ45706
  • FLJ59041
  • nucleolin
  • Protein C23

Background

Nucleolin (NCL, also called Protein C23) is a major phosphoprotein present in nucleolus of growing eukaryotic cells which is implicated in the synthesis as well as maturation of ribosomes. Localized mainly to nucleolus, NCL may also be found in cytoplasmic mRNP granules containing untranslated mRNAs (mRNP granule complex contains - NCL, ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs). NCL interacts with APTX, NSUN2, TERT, ERBB4, GZF1, GZF1, NVL and C1QBP, and is a part of SWAP complex (NPM1, NCL, PARP1 and SWAP70) as well as another complex containing NCL, HTATSF1/Tat-SF1, CDK9 and CCNT1, RNA polymerase II, SUPT5H. NCL may be found associated with intranucleolar chromatin and pre-ribosomal particles where it induces chromatin de-condensation via binding to histone H1 and play a key role in pre-rRNA transcription as well as ribosome assembly. NCL has also been suggested to implicate in the process of transcriptional elongation and it binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Besides being essential to rRNA processing in nucleolus, NCL has several other functions such as transcription regulation, modulation of mRNA stability and DNA replication and recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-241
Species: Hu, Mu, Rt, Pl
Applications: WB, Simple Western, ChIP, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IHC-WhMt, KD

Publications for Nucleolin Antibody (NB600-241)(22)

We have publications tested in 5 confirmed species: Human, Mouse, Human , Plant, Rat .

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-Fr, IM, WB.


Filter By Application
ChIP
(4)
ICC/IF
(6)
IHC
(1)
IHC-Fr
(1)
IM
(1)
WB
(8)
All Applications
Filter By Species
Human
(8)
Mouse
(6)
Human
(1)
Plant
(1)
Rat
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB600-241 Applications Species
Wu R, Li L, Bai Y et al. The long noncoding RNA LUCAT1 promotes colorectal cancer cell proliferation by antagonizing Nucleolin to regulate MYC expression Cell Death Dis Oct 23 2020 [PMID: 33097685] (ChIP) ChIP
Podratz JL, Tang JJ, Polzin MJ et al. Mechano growth factor interacts with nucleolin to protect against cisplatin-induced neurotoxicity Exp. Neurol. Jun 5 2020 [PMID: 32511954] (ICC/IF, Mouse) ICC/IF Mouse
Okur MN, Lee JH, Osmani W et al. Cockayne syndrome group A and B proteins function in rRNA transcription through nucleolin regulation Nucleic Acids Res. Jan 23 2020 [PMID: 31970402] (ChIP, Human) ChIP Human
Hoffmann F, Umbreit C, Kruger T et al. Identification of Proteomic Markers in Head and Neck Cancer Using MALDI-MS Imaging, LC-MS/MS, and Immunohistochemistry. Prot. Clin. Appl. Nov 26 2018 [PMID: 30411850] (IHC-Fr, Human) IHC-Fr Human
Banerjee S, Aponte-Diaz D, Yeager C et al. Hijacking of Multiple Phospholipid Biosynthetic Pathways and Induction of Membrane Biogenesis by a Picornaviral 3CD Protein bioRxiv 2017 Oct 29 [PMID: 29782554] (ICC/IF, Human) ICC/IF Human
Park OH, Park J, Yu M et al. Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay. Genes Dev. Sep 15 2016 [PMID: 27798850] (WB, Human) WB Human
Corne TD, Sieprath T, Vandenbussche J et al. Uncoordinated regulation of focal adhesions and cytoskeletal tension due to the loss of a-type lamins. Cell Adh Migr Oct 28 2016 [PMID: 27791462] (WB, Human) WB Human
Robijns J, Molenberghs F, Sieprath T et al. In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells. Sci Rep. Jul 27 2016 [PMID: 27461848] (WB) WB
Leaderer D, Cashman SM, Kumar-Singh R. Topical application of a G-Quartet aptamer targeting nucleolin attenuates choroidal neovascularization in a model of age-related macular degeneration. Exp. Eye Res. 2015 Sep 12 [PMID: 26368850] (IHC, Mouse) IHC Mouse
Sieprath T, Corne TD, Nooteboom M et al. Sustained accumulation of prelamin A and depletion of lamin A/C both cause oxidative stress and mitochondrial dysfunction but induce different cell fates. Nucleus 2015 May 21 [PMID: 25996284] (WB) WB
Show All 22 Publications.

Reviews for Nucleolin Antibody (NB600-241) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 review tested in 1 species: Human.

Reviews using NB600-241:
Filter by Applications
ICC
(1)
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Nucleolin NB600-241
Enlarge
5
reviewed by:
Anonymous
ICC Human 02/09/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman prostate cancer cells
SpeciesHuman
LotC-3
Immunofluorescence Nucleolin NB600-241
Enlarge
5
reviewed by:
Anonymous
IF 08/22/2012
View

Summary

ApplicationImmunofluorescence
Special ApplicationsThe antibody was used for immunofluorescent staining of nucleolin in MCF-7 breast cancer cells followed by confocal microscopy.
Pub Med ID22693611

Product General Protocols

View specific protocols for Nucleolin Antibody (NB600-241): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Nucleolin Antibody (NB600-241). (Showing 1 - 1 of 1 FAQs).

  1. Have you tested NB600-241 on nucleus fraction or cytoplasmic fraction? Do you have any publication or data could support the signal only appear on nucleus?
    • Yes, it has been tested in nuclear extracts. Furthermore, it appears that a customer detected nucleolin in both the cytomplasm and the nucleus. Nucleolin appears to reside in both the cytoplasm and the nucleus (UniProt P19338) so it would be impossible to create an antibody that only detects nucleolin in just the nucleus. Our 10+ publications using this antibody can also be found on our website. These papers may provide further insight beyond our internal testing.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Nucleolin Antibody (NB600-241)

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Diseases for Nucleolin Antibody (NB600-241)

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Blogs on Nucleolin.

Nucleolin: A Multifaceted Nucleolar Phosphoprotein
Nucleolin is a ubiquitous, nonhistone nucleolar phosphoprotein of exponentially growing eukaryotic cells and is present in abundance at the dense fibrillar and granular regions of nucleolus. Intact nucleolin is the major species and represents 5% of n...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

Nucleolin: To the Nucleus and Beyond!
Nucleolin is a multifunctional phosphoprotein ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. Nucleolin has a bipartite nuclear localization signal sequence and is conserved across the species. Nucleolin levels are expres...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
02/09/2017
Application: ICC
Species: Human

Anonymous
08/22/2012
Application: IF
Species:

Bioinformatics

Gene Symbol NCL
Entrez
Uniprot