NPIP Antibody - BSA Free

Western Blot: NPIPB3 Antibody [NBP2-46813] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4

Immunocytochemistry/Immunofluorescence: NPIPB3 Antibody [NBP2-46813] - Staining of human cell line A-431 shows localization to nucleoplasm.

Immunohistochemistry: NPIPB3 Antibody [NBP2-46813] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NPIP Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NRMGHQPPPPTQQHSIIDNSLSLKTP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPIP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  NPIP Recombinant Protein Antigen (NBP2-46813PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NPIP Antibody - BSA Free

• FLJ42525
• FLJ44848
• nuclear pore complex interacting protein
• nuclear pore complex-interacting protein

Background

The nuclear pore complex (NPC) is a very large structure made up of at least 50 different proteins that span the double membrane of the nuclear envelope functioning as a gateway for macromolecular traffic between the cytoplasm and the nucleus (Nakielny and Dreyfuss,1999). Discrete nuclear pore complex proteins or nucleoporins such as NUP98, NUP180 and p62 have been implicated in autoimmune disease and cancer. Patients with primary biliary cirrhosis (PBC) frequently produce autoantibodies against p62 and NUP180 (Wilken et al., 1993; Nesher et al., 2001) while NUP98 translocations have been found in patients with acute myelogenous leukemia (AML) (Jaju et al. 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NPIP
• Entrez:
  • 642778(Human)