MRP6 Antibody (M6II-7)

• Reactivity: Hu
• Applications: WB, Flow, IHC, IHC-Fr
• Clone: M6II-7
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

MRP6 Antibody (M6II-7) Summary

• Immunogen: The antibody reacts with the MRP6 antigen.
• Specificity: M6II-7 reacts with MRP6, a 190-200 kD transmembrane protein that is related to the multidrug resistance related protein MRP. Mutations in the MRP6 gene are responsible for the connective tissue disorder Pseudoxanthoma elasticum (PXE). M6II-7 was raised against a bacterial fusion protein of human MRP6, containing amino acids 764-964, spanning the putative 12th transmembrane region as well as predicted internal and external regions of the protein. M6II-7 did not cross-react with the human MDR1, MRP1, MRP2, MRP3, MRP4, MRP5 gene products.
• Isotype: IgG2 Alpha
• Clonality: Monoclonal
• Host: Rat
• Gene: ABCC6
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: M6II-7 can be used to detect human MRP6 in cells and tissues. Immunocytochemistry: use 1:20-50 dilution on acetone fixed cytospin preparations. For immunohistochemistry: M6II-7 (use 1:20-50) on acetone fixed frozen sections can be followed by incubation w

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1.0% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for MRP6 Antibody (M6II-7)

• Anthracycline resistance-associated protein
• ARAPXE
• ATP-binding cassette, sub-family C (CFTR/MRP), member 6
• EST349056
• MLP1ABC34
• MOATE
• MOAT-E
• MRP6ATP-binding cassette sub-family C member 6
• multidrug resistance-associated protein 6
• Multi-specific organic anion transporter E
• pseudoxanthoma elasticum
• PXE1
• URG7

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). The encoded protein, a member of the MRP subfamily, is involved in multi-drug resistance. Mutations in this gene cause pseudoxanthoma elasticum. Alternatively spliced transcript variants that encode different proteins have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRP6 Antibody (NBP1-42342)(1)

Publication using NBP1-42342

• Roggenbeck BA, Carew MW, Charrois GJ et al. Characterization of Arsenic Hepatobiliary Transport Using Sandwich Cultured Human Hepatocytes. Toxicol. Sci. 2015 Mar 09 [PMID: 25752797] (WB, Human)

Bioinformatics

• Gene Symbol: ABCC6