p130Cas/BCAR1 Antibody (OTI1A9)

Images

 
Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1.
Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody.
Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BCAR1 antibody, and then analyzed ...read more
Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BCAR1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BCAR1 mouse monoclonal antibody.
Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - Analysis of Hela cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - Analysis of Jurkat cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI1A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.53 mg/ml

Order Details

p130Cas/BCAR1 Antibody (OTI1A9) Summary

Immunogen
Full length human recombinant protein of human BCAR1 (NP_055382) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BCAR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
93.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.53 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p130Cas/BCAR1 Antibody (OTI1A9)

  • BCAR1
  • breast cancer estrogen resistance 1
  • Cas scaffolding protein family member 1
  • CAS1
  • CASFLJ45059
  • CASS1
  • CASS1CRKASbreast cancer estrogen resistance protein 1
  • CRKAS
  • Crk-associated substrate p130Cas
  • CRK-associated substrate
  • FLJ12176
  • p130Cas

Background

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BCAR1
Entrez