Nox4 Antibody [Unconjugated]

Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Nox4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8158) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nox4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Nox4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Nox4
  Lys450-Ser578
  Accession # Q9NPH5
• Specificity: Detects human Nox4 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NOX4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nox4 Antibody [Unconjugated]

• EC 1.6.3
• Kidney oxidase-1
• Kidney superoxide-producing NADPH oxidase
• KOX
• KOX-1
• KOX-1Renal NAD(P)H-oxidase
• NADPH oxidase 4
• Nox4
• RENOX
• RENOXEC 1.6.3.-

Background

NADPH Oxidase 4/NOX4, also known as Renal NADPH Oxidase/RENOX and Kidney Oxidase 1/KOX1, is a 66 to 75 kDa member of the NOX family of NADPH oxidases.  NOX family members are a major source of reactive oxygen species (ROS), including hydrogen peroxide and superoxide, and are critical mediators of redox signaling.  NOX4 is strongly expressed in the kidney, especially in the proximal convoluted tubule cells of the renal cortex, and may function as an oxygen sensor that regulates the synthesis of erythropoietin.  Expression is also high in endothelial cells, and because aortas from deficient mice exhibit increased inflammation, hypertrophy and endothelial dysfunction, NOX4 may function to protect the vasculature during ischemic or inflammatory stress.  Human NOX4 is 578 amino acids (aa) in length, with several additional smaller isoforms identified.  Over aa 450-578, human NOX4 has 92% sequence identity with mouse NOX4, and 91% identity with rat NOX4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nox4 Antibody (AF8158)(2)

Publications using AF8158

• X Zhang, H Liu, JQ Zhou, S Krick, JW Barnes, VJ Thannickal, YY Sanders Modulation of H4K16Ac levels reduces pro-fibrotic gene expression and mitigates lung fibrosis in aged mice Theranostics, 2022-01-01;12(2):530-541. 2022-01-01 [PMID: 34976199] (Western Blot, Human)
• YY Sanders, X Lyu, QJ Zhou, Z Xiang, D Stanford, S Bodduluri, SM Rowe, VJ Thannickal Brd4-p300 inhibition down-regulates Nox4 and accelerates lung fibrosis resolution in aged mice JCI Insight, 2020-07-23;0(0):. 2020-07-23 [PMID: 32544088] (Western Blot, Human)

Review for Nox4 Antibody (AF8158) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 11/24/2017

Summary

• Application: Western Blot
• Sample Tested: C2C12 mouse myoblast cell line,Differentiated C2C12 myoblast cells
• Species: Mouse
• Lot: CGDK021706A

Comments

• Comments: Anti-NOX4 - 1:500
  Anti-sheep HRP - 1:1000

Bioinformatics

• Gene Symbol: NOX4
• Uniprot:
  • Q9NPH5()