Nox4 Antibody [Alexa Fluor® 532]

• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

Nox4 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human Nox4
  Lys450-Ser578
  Accession # Q9NPH5
• Specificity: Detects human Nox4 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

NADPH Oxidase 4/NOX4, also known as Renal NADPH Oxidase/RENOX and Kidney Oxidase 1/KOX1, is a 66 to 75 kDa member of the NOX family of NADPH oxidases.  NOX family members are a major source of reactive oxygen species (ROS), including hydrogen peroxide and superoxide, and are critical mediators of redox signaling.  NOX4 is strongly expressed in the kidney, especially in the proximal convoluted tubule cells of the renal cortex, and may function as an oxygen sensor that regulates the synthesis of erythropoietin.  Expression is also high in endothelial cells, and because aortas from deficient mice exhibit increased inflammation, hypertrophy and endothelial dysfunction, NOX4 may function to protect the vasculature during ischemic or inflammatory stress.  Human NOX4 is 578 amino acids (aa) in length, with several additional smaller isoforms identified.  Over aa 450-578, human NOX4 has 92% sequence identity with mouse NOX4, and 91% identity with rat NOX4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.