Notch-1 Antibody

Images

 
Western Blot: Notch-1 Antibody [NB300-251] - Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17beta-estradiol. Lane 3: MCF-7 + 10 uM gamma secretase inhibitor. Load: 35 ug per lane. Primary antibody: Notch1 antibody ...read more
Immunohistochemistry: Notch-1 Antibody [NB300-251] - Analysis of Tissue: Exocrine glands of human pancreas Fixation: FFPE Primary antibody: Notch1 antibody at 1:200 Staining: moderate to strong membranous staining and ...read more
Western Blot: Notch-1 Antibody [NB300-251] - Notch 1 Antibody [NB 300-251]- at 1:500, against myc-tagged transiently transfected mouse Notch constructs in 293 cells.
Western Blot: Notch-1 Antibody [NB300-251] - Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular ...read more
Western Blot: Notch-1 Antibody [NB300-251] - Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Notch-1 Antibody Summary

Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch-1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD). (Uniprot: P46531)
Epitope
The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Specificity
Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form (NICD).
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
NOTCH1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:20000-1:60000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
Application Notes
This antibody is reactive with Notch-1 by western blotting, Immunohistochemistry (IHC) and ELISA. An 80 kDa band corresponding to Notch 1 was observed at a 1:500 dilution. Specific conditions for reactivity should be optimized by the end user.
Theoretical MW
272.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Notch-1 Overexpression Lysate
Control Peptide
Notch-1 Peptide (NB300-251PEP)
Reviewed Applications
Read 1 Review rated 1
using
NB300-251 in the following applications:

Publications
Read Publications using
NB300-251 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% Sodium Azide
Purity
Unpurified

Notes

This antiserum is directed against human Notch-1

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Notch-1 Antibody

  • EC 2.1.2.11
  • EC 3.4.21.68
  • hN1
  • Notch (Drosophila) homolog 1 (translocation-associated)
  • notch 1Notch homolog 1, translocation-associated (Drosophila)
  • Notch homolog 1, translocation-associated
  • Notch1
  • Notch-1
  • TAN1
  • TAN1neurogenic locus notch homolog protein 1
  • Translocation-associated notch protein TAN-1

Background

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-251
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for Notch-1 Antibody (NB300-251)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
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Human
(3)
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Showing Publications 1 - 9 of 9.
Publications using NB300-251 Applications Species
Han J, Hyun J, Park J et al. Aberrant role of pyruvate kinase M2 in the regulation of gamma-secretase and memory deficits in Alzheimer's disease Cell reports Dec 7 2021 [PMID: 34879266]
Bhardwaj M, Sharma A, Sen S et al. Chlamydia and ocular adnexal lymphomas: An Indian experience Exp. Mol. Pathol. 2016 Jul 17 [PMID: 27435913] (WB, Human) WB Human
Lee HA, Park S, Kim Y. Effect of beta-carotene on cancer cell stemness and differentiation in SK-N-BE(2)C neuroblastoma cells. Oncol Rep 2013 Oct [PMID: 23900747]
Park S, Kim J, Kim Y. Mulberry leaf extract inhibits cancer cell stemness in neuroblastoma Nutr Cancer 2012 Aug [PMID: 22860924] (WB, Human) WB Human
Sciacca S, Pilato M, Mazzoccoli G et al. Anti-correlation between longevity gene SirT1 and Notch signaling in ascending aorta biopsies from patients with bicuspid aortic valve disease Heart Vessels 2012 Feb 28 [PMID: 22370592] (WB, Human) WB Human
Wang J, Zhang Y, Wei J et al. Effect of Notch activation on the regenerative response to acute renal failure. Am J Physiol Renal Physiol 2010 Jan [PMID: 19828677]
Solomon A, Mian Y, Ortega-Cava C et al. Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants. Dev Biol 2008 Apr [PMID: 18334253]
Stylianou S, Clarke RB, Brennan K et al. Aberrant activation of notch signaling in human breast cancer. Cancer Res 2006 Feb [PMID: 16452208]
Milano, J et al. Modulation of Notch Processing by gamma-Secretase Inhibitors Causes Intestinal Goblet Cell Metaplasia Induction of Genes Known to Specify Gut Secretory Lineage Differentiation. Toxicological Sciences. [PMID: 15319485]

Review for Notch-1 Antibody (NB300-251) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB300-251:
Filter by Applications
WB
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot Notch-1 NB300-251
Enlarge
1
reviewed by:
Ram Kuwar
WB Rat 10/23/2020
View

Summary

ApplicationWestern Blot
Sample TestedRat brain tissue
SpeciesRat
Lot42173

Comments

Comments*Novus Response: Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Notch-1 Antibody (NB300-251). (Showing 1 - of FAQs).

    Control Lysate(s)

    Secondary Antibodies

     

    Isotype Controls

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    Recent Reviews

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    1

    Ram Kuwar
    10/23/2020
    Application: WB
    Species: Rat

    Bioinformatics

    Gene Symbol NOTCH1
    Entrez
    Uniprot