Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free

Flow Cytometry: Notch-1 Antibody (E6) - Chimeric [NBP2-62557] - Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (NBP2-62557, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunocytochemistry /Immunofluorescence: Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free [NBP2-62557] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-62557 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62557, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu
• Applications: ELISA, Flow, ICC/IF, IHC
• Clone: E6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: This antibody was selected against a fusion protein consisting of EGF domains 1-12 of murine Notch1 fused to a human Fc domain and binds to to the NRR of mouse Notch1.
• Specificity: An engineered ScFv fragment antibody that binds to both mouse mNRR1 and human hNRR1 but not NRR2.
• Isotype: IgG Lambda
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NOTCH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:1000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original human scFv format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free

• EC 2.1.2.11
• EC 3.4.21.68
• hN1
• Notch (Drosophila) homolog 1 (translocation-associated)
• notch 1Notch homolog 1, translocation-associated (Drosophila)
• Notch homolog 1, translocation-associated
• Notch1
• Notch-1
• TAN1
• TAN1neurogenic locus notch homolog protein 1
• Translocation-associated notch protein TAN-1

Background

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Notch-1 Antibody (NBP2-62557)(1)

Publication using NBP2-62557

• Ashish Tyagi, Balaji Chandrasekaran, Venkatesh Kolluru, Becca V Baby, Cibi A. Sripathi, Murali K. Ankem, Srinivasa R. Ramisetti, Venkat R. Chirasani, Nikolay V Dokholyan, Arun K. Sharma, Chendil Damodaran ASR490, a Small Molecule, Overrides Aberrant Expression of Notch1 in Colorectal Cancer Molecular cancer therapeutics 2023-12-04 [PMID: 33087513]

FAQs for Notch-1 Antibody (NBP2-62557). (Showing 1 - of FAQ).

Bioinformatics

• Gene Symbol: NOTCH1