NOMO Antibody

Western blot shows lysates of LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human NOMO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NOMO at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NOMO Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NOMO
  Ser32- Asp1155
  Accession # Q15155
• Specificity: Detects human NOMO in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NOMO2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NOMO Antibody

• NOMO
• NOMO2
• PM5

Background

NOMO, also known as pM5, is a 130 kDa type I membrane protein with no recognizable functional motif. It is a binding partner of Nicalin, a nicastin-related protein. The Nicalin/NOMO complex is localized in the ER and functions as an antagonist for Nodal and Activin signaling. Three copies of the NOMO gene, encoding NOMO isoforms 1, 2 and 3 have been described. The extracellular domains (ECD) of the isoforms share 99% amino acid sequence homology. The ECD of human and mouse NOMO also share 94% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOMO2
• Uniprot:
  • Q15155()