Nicastrin Antibody (716918)

Images

 
Nicastrin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Nicastrin Monoclonal Antibody (Catalog # MAB5378) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
716918
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Nicastrin Antibody (716918) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Nicastrin
Asn34-Glu669
Accession # Q92542
Specificity
Detects human Nicastrin in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NCSTN
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB5378 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nicastrin Antibody (716918)

  • anterior pharynx-defective 2
  • APH2
  • ATAG1874
  • KIAA0253APH2
  • NCSTN
  • Nicastrin

Background

Nicastrin (NCT) is a 150‑160 kDa member of the Nicastrin family of proteins. It is a component of the aspartyl protease gamma -secretase complex and serves to stabilize and direct gamma -secretase components to proper positions in the plasma membrane. The gamma -secretase complex mediates the cleavage of intramembrane proteins such as Notch-1 and APP. Mature human Nicastrin is a 676 amino acid type I transmembrane glycoprotein. It contains a 636 aa extracellular domain (aa 34‑669) that shows a 58 aa sequence (aa 312‑369) which interacts with gamma -secretase substrates. There are multiple splice variants of NCT. One shows a deletion of aa 195‑322 and 394‑709, a second shows a 29 aa substitution for the C-terminal 604 aa and a third shows a deletion of aa 200‑709 accompanied by an insertion of 33 aa after Leu30. Over aa 34‑669, human NCT shares 90% aa identity with mouse NCT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-74510
Species: Hu, Mu, Pm, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP2-75632
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-P, WB
NB100-74360
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-13075
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
MAB931
Species: Hu, Mu
Applications: CyTOF-ready, IHC, ICFlow, WB
NB100-74513
Species: Hu, Mu, Pm, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP1-90813
Species: Hu
Applications: IHC, IHC-P, WB
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
NB100-78486
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
H00004782-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
NBP1-81000
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, WB
NBP1-81406
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15039
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-P, WB
H00114799-T01
Species: Hu
Applications: WB

Publications for Nicastrin Antibody (MAB5378) (0)

There are no publications for Nicastrin Antibody (MAB5378).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for Nicastrin Antibody (MAB5378) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB5378:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Nicastrin MAB5378
Enlarge
5
reviewed by:
Ashleigh Dean
ICC Human 03/22/2022
View

Summary

ApplicationImmunocytochemistry
Sample TestedHepG2 human hepatocellular carcinoma cell line
SpeciesHuman
Lot716918

Comments

CommentsIncubated at 10 ug/mL for 3 hours at room temperature

FAQs for Nicastrin Antibody (MAB5378) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Nicastrin Products

Bioinformatics Tool for Nicastrin Antibody (MAB5378)

Discover related pathways, diseases and genes to Nicastrin Antibody (MAB5378). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nicastrin Antibody (MAB5378)

Discover more about diseases related to Nicastrin Antibody (MAB5378).
 

Pathways for Nicastrin Antibody (MAB5378)

View related products by pathway.

PTMs for Nicastrin Antibody (MAB5378)

Learn more about PTMs related to Nicastrin Antibody (MAB5378).

Blogs on Nicastrin

There are no specific blogs for Nicastrin, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ashleigh Dean
03/22/2022
Application: ICC
Species: Human

Bioinformatics

Gene Symbol NCSTN
Uniprot