Human IL-6R alpha Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-6R alpha Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-6 sR Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-6 sR in cell culture supernates, serum, plasma, and urine. It contains recombinant human IL-6 sR produced in Sf 21 cells and antibodies raised against this protein. This kit has been shown to accurately quantitate the recombinant soluble IL-6 R. Results obtained using natural IL-6 s...R showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-6 sR. Since the measurement of human IL-6 sR by this immunoassay is insensitive to added recombinant human IL-6, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples ( i.e. the total amount of free receptor plus the total amount of receptor bound to IL-6).
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• Specificity: Natural and recombinant human IL-6 sR
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL6R

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-6R alpha Quantikine ELISA Kit

• CD126
• gp80
• IL-6 R alpha
• IL6Q
• IL6R alpha
• IL-6R alpha
• IL6R
• IL-6R-1
• IL6RA
• IL-6Ra
• IL6RQ
• interleukin 6 receptor

Background

IL-6 R alpha is a transmembrane subunit of the receptor for Interleukin-6. It associates with gp130 which is a shared component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. IL-6 plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-6R alpha (DR600)(46)

Publications using DR600

• Stukas, S;Goshua, G;Conway, EM;Lee, AYY;Hoiland, RL;Sekhon, MS;Y C Chen, L; ABO blood group and COVID-19 severity: Associations with endothelial and adipocyte activation in critically ill patients PloS one 2025-04-02 [PMID: 40173171] (Human)
• Odell, ID;Agrawal, K;Sefik, E;Odell, AV;Caves, E;Kirkiles-Smith, NC;Horsley, V;Hinchcliff, M;Pober, JS;Kluger, Y;Flavell, RA; IL-6 trans-signaling in a humanized mouse model of scleroderma Proceedings of the National Academy of Sciences of the United States of America 2023-09-12 [PMID: 37669366] (Human)
• V Bratseth, J Nordeng, R Helseth, S Solheim, S Åkra, H Arnesen, G Chiva-Blan, I Seljeflot Circulating Microvesicles in Association with the NLRP3 Inflammasome in Coronary Thrombi from STEMI Patients Biomedicines, 2022-09-05;10(9):. 2022-09-05 [PMID: 36140297] (Human)
• J Baran, ? Niewiara, J Podolec, M Siedli?ski, E Józefczuk, A Bernacik, R Badacz, T Przew?ocki, P Pieni??ek, K ?mudka, J Legutko, A Kab?ak-Zie Serum and Vascular Stiffness Biomarkers Associated with the Severity of Degenerative Aortic Valve Stenosis and Cardiovascular Outcomes Oncogene, 2022-06-17;9(6):. 2022-06-17 [PMID: 35735822] (Human)
• S Siamakpour, F Cao, J Lyu, Y Ren, AB Nixon, J Xie, AT Bush, MD Starr, JR Bain, MJ Muehlbauer, O Ilkayeva, V Byers Krau, JL Huebner, NJ Chao, AD Sung Evaluating immune response and metabolic related biomarkers pre-allogenic hematopoietic stem cell transplant in acute myeloid leukemia PLoS ONE, 2022-06-14;17(6):e0268963. 2022-06-14 [PMID: 35700185] (Human)
• R Salemi, G Gattuso, B Tomasello, A Lavoro, A Gaudio, M Libra, SS Signorelli, S Candido Co-Occurrence of Interleukin-6 Receptor Asp358Ala Variant and High Plasma Levels of IL-6: An Evidence of IL-6 Trans-Signaling Activation in Deep Vein Thrombosis (DVT) Patients Biomolecules, 2022-05-10;12(5):. 2022-05-10 [PMID: 35625609] (Human)
• L Suo, M Wang Dexmedetomidine attenuates oxygen-glucose deprivation/ reperfusion-induced inflammation through the miR-17-5p/ TLR4/ NF-kappaB axis BMC anesthesiology, 2022-04-29;22(1):126. 2022-04-29 [PMID: 35488217] (Rat)
• CD Ene, SR Georgescu, M Tampa, C Matei, CI Mitran, MI Mitran, MN Penescu, I Nicolae Cellular Response against Oxidative Stress, a Novel Insight into Lupus Nephritis Pathogenesis Journal of personalized medicine, 2021-07-22;11(8):. 2021-07-22 [PMID: 34442337] (Human)
• J Nordeng, H Schandiz, S Solheim, S Åkra, P Hoffman, B Roald, B Bendz, H Arnesen, R Helseth, I Seljeflot The Inflammasome Signaling Pathway Is Actively Regulated and Related to Myocardial Damage in Coronary Thrombi from Patients with STEMI Mediators of Inflammation, 2021-05-27;2021(0):5525917. 2021-05-27 [PMID: 34135690] (Human)
• CD Ene, MN Penescu, SR Georgescu, M Tampa, I Nicolae Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma Metabolites, 2020-12-27;11(1):. 2020-12-27 [PMID: 33375435] (Human)
• KP Loh, JA Tooze, BJ Nicklas, SB Kritchevsk, JD Williamson, LR Ellis, BL Powell, TS Pardee, NG Goyal, HD Klepin Inflammatory biomarkers, geriatric assessment, and treatment outcomes in acute myeloid leukemia J Geriatr Oncol, 2019-04-05;0(0):. 2019-04-05 [PMID: 30962090] (Human)
• S Ishikawa, M Tasaki, T Kuroda, D Kobayashi, K Saito, Y Nakagawa, M Ikeda, K Takahashi, Y Tomita Management of Juvenile Idiopathic Arthritis in ABO-incompatible Kidney Transplantation: A Case Report Transplant. Proc., 2018-04-01;50(3):869-872. 2018-04-01 [PMID: 29661455] (Human)
• H Munir, LS Ward, L Sheriff, S Kemble, S Nayar, F Barone, GB Nash, HM McGettrick Adipogenic differentiation of MSC alters their immunomodulatory properties in a tissue-specific manner Stem Cells, 2017-04-24;0(0):. 2017-04-24 [PMID: 28376564] (Human)
• Koji Osuka Inhibitory Mechanism of the Outer Membrane Growth of Chronic Subdural Hematomas J. Neurotrauma, 2017-02-27;0(0):. 2017-02-27 [PMID: 28027695] (Human)
• Prevalent peripheral arterial disease and inflammatory burden BMC Geriatr, 2016-12-09;16(1):213. 2016-12-09 [PMID: 27938334] (Human)
• IL-6 trans-signaling is another pathway to upregulate Osteopontin Cytokine, 2016-11-15;90(0):88-95. 2016-11-15 [PMID: 27863335] (Human)
• Inflammatory Markers and the Risk of Hip and Vertebral Fractures in Men: the Osteoporotic Fractures in Men (MrOS) J Bone Miner Res, 2016-07-25;0(0):. 2016-07-25 [PMID: 27371811] (Human)
• P Srikanth, R F Chun, M Hewison, J S Adams, R Bouillon, D Vandersch, N Lane, P M Cawthon, T Dam, E Barrett-C, L B Daniels, J M Shikany, M L Stefanick, J A Cauley, E S Orwoll, C M Nielson Associations of total and free 25OHD and 1,25(OH)2D with serum markers of inflammation in older men. Osteoporosis International : a Journal Established as Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the Usa, 2016-02-23;0(0):1433-2965. 2016-02-23 [PMID: 26905270] (Human)
• Zhong H, Davis A, Ouzounova M, Carrasco R, Chen C, Breen S, Chang Y, Huang J, Liu Z, Yao Y, Hurt E, Moisan J, Fung M, Tice D, Clouthier S, Xiao Z, Wicha M, Korkaya H, Hollingsworth R A Novel IL6 Antibody Sensitizes Multiple Tumor Types to Chemotherapy Including Trastuzumab-Resistant Tumors. Cancer Res, 2016-01-07;76(2):480-90. 2016-01-07 [PMID: 26744529] (Human)
• Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, Usami I, Sakamoto S, Abe H, Watanabe T, Yamori T, Shibasaki M, Nomoto A Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFalpha-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction. PLoS ONE, 2015-03-18;10(3):e0119415. 2015-03-18 [PMID: 25785838] (Human)
• Csaszar , Elizabet, Wang , Weijia, Usenko , Tatiana, Qiao , Wenlian, Delaney , Colleen, Bernstein , Irwin D, Zandstra , Peter W Blood stem cell fate regulation by Delta-1-mediated rewiring of IL-6 paracrine signaling. Blood, 2013-11-15;123(5):650-8. 2013-11-15 [PMID: 24243972] (Human)
• Salsano , Ettore, Rizzo , Ambra, Bedini , Gloria, Bernard , Loris, Dall'olio , Valentin, Volorio , Sara, Lazzaroni , Monica, Ceccherini , Isabella, Lazarevic , Dejan, Cittaro , Davide, Stupka , Elia, Paterra , Rosina, Farina , Laura, Savoiardo , Mario, Pareyson , Davide, Sciacca , Francesc An autoinflammatory neurological disease due to interleukin 6 hypersecretion. J Neuroinflammation, 2013-02-21;10(0):29. 2013-02-21 [PMID: 23432807] (Human)
• Tai Y, Chang B, Kong S, Fulciniti M, Yang G, Calle Y, Hu Y, Lin J, Zhao J, Cagnetta A, Cea M, Sellitto M, Zhong M, Wang Q, Acharya C, Carrasco D, Buggy J, Elias L, Treon S, Matsui W, Richardson P, Munshi N, Anderson K Bruton tyrosine kinase inhibition is a novel therapeutic strategy targeting tumor in the bone marrow microenvironment in multiple myeloma. Blood, 2012-06-11;120(9):1877-87. 2012-06-11 [PMID: 22689860] (Human)
• Barbour KE, Boudreau R, Danielson ME, Youk AO, Wactawski-Wende J, Greep NC, LaCroix AZ, Jackson RD, Wallace RB, Bauer DC, Allison MA, Cauley JA Inflammatory markers and the risk of hip fracture: the Women's Health Initiative. J. Bone Miner. Res., 2012-05-01;27(5):1167-76. 2012-05-01 [PMID: 22392817] (Human)
• Holmlund U, Amoudruz P, Johansson MA, Haileselassie Y, Ongoiba A, Kayentao K, Traore B, Doumbo S, Schollin J, Doumbo O, Montgomery SM, Sverremark-Ekstrom E Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring. Clin. Exp. Immunol., 2010-10-05;162(3):500-9. 2010-10-05 [PMID: 20942805] (Human)
• Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria. Transplantation, 2009-11-15;88(9):1109-16. 2009-11-15 [PMID: 19898207] (Human)
• Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target. Cancer Cell, 2009-10-06;16(4):309-23. 2009-10-06 [PMID: 19800576] (Human)
• Fuligni AJ, Telzer EH, Bower J, Irwin MR, Kiang L, Cole SW Daily family assistance and inflammation among adolescents from Latin American and European backgrounds. Brain Behav. Immun., 2009-03-09;23(6):803-9. 2009-03-09 [PMID: 19275931] (Human)
• Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells. Cancer Res., 2009-01-01;69(1):329-37. 2009-01-01 [PMID: 19118018] (Human)
• Perez-Andres M, Almeida J, Martin-Ayuso M, De Las Heras N, Moro MJ, Martin-Nunez G, Galende J, Cuello R, Abuin I, Moreno I, Dominguez M, Hernandez J, Mateo G, San Miguel JF, Orfao A Soluble and membrane levels of molecules involved in the interaction between clonal plasma cells and the immunological microenvironment in multiple myeloma and their association with the characteristics of the disease. Int. J. Cancer, 2009-01-15;124(2):367-75. 2009-01-15 [PMID: 19003959] (Human)
• Egler RA, Burlingame SM, Nuchtern JG, Russell HV Interleukin-6 and soluble interleukin-6 receptor levels as markers of disease extent and prognosis in neuroblastoma. Clin. Cancer Res., 2008-11-01;14(21):7028-34. 2008-11-01 [PMID: 18980999] (Human)
• Shariat SF, Karam JA, Walz J, Roehrborn CG, Montorsi F, Margulis V, Saad F, Slawin KM, Karakiewicz PI Improved prediction of disease relapse after radical prostatectomy through a panel of preoperative blood-based biomarkers. Clin. Cancer Res., 2008-06-15;14(12):3785-91. 2008-06-15 [PMID: 18559597] (Human)
• Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy. AIDS Res. Hum. Retroviruses, 2007-07-01;23(7):886-90. 2007-07-01 [PMID: 17678471] (Human)
• Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils. Blood, 2007-06-13;110(6):1748-55. 2007-06-13 [PMID: 17567983] (Human)
• Cauley JA, Danielson ME, Boudreau RM, Forrest KY, Zmuda JM, Pahor M, Tylavsky FA, Cummings SR, Harris TB, Newman AB Inflammatory markers and incident fracture risk in older men and women: the Health Aging and Body Composition Study. J. Bone Miner. Res., 2007-07-01;22(7):1088-95. 2007-07-01 [PMID: 17419681] (Human)
• Richards PJ, Nowell MA, Horiuchi S, McLoughlin RM, Fielding CA, Grau S, Yamamoto N, Ehrmann M, Rose-John S, Williams AS, Topley N, Jones SA Functional characterization of a soluble gp130 isoform and its therapeutic capacity in an experimental model of inflammatory arthritis. Arthritis Rheum., 2006-05-01;54(5):1662-72. 2006-05-01 [PMID: 16646038] (Human)
• Tsai MY, Hanson NQ, Straka RJ, Hoke TR, Ordovas JM, Peacock JM, Arends VL, Arnett DK Effect of influenza vaccine on markers of inflammation and lipid profile. J. Lab. Clin. Med., 2005-06-01;145(6):323-7. 2005-06-01 [PMID: 15976761] (Human)
• Kaneyama K, Segami N, Sun W, Sato J, Fujimura K Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005-04-01;99(4):411-8. 2005-04-01 [PMID: 15772591] (Human)
• Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection. Transpl. Int., 2005-02-01;18(2):177-85. 2005-02-01 [PMID: 15691270] (Human)
• Hirota H, Izumi M, Hamaguchi T, Sugiyama S, Murakami E, Kunisada K, Fujio Y, Oshima Y, Nakaoka Y, Yamauchi-Takihara K Circulating interleukin-6 family cytokines and their receptors in patients with congestive heart failure. Heart Vessels, 2004-09-01;19(5):237-41. 2004-09-01 [PMID: 15372299] (Human)
• Petrucci MT, Ricciardi MR, Gregorj C, Ciapponi L, Savino R, Ciliberto G, Tafuri A Effects of IL-6 variants in multiple myeloma: growth inhibition and induction of apoptosis in primary cells. Leuk. Lymphoma, 2002-12-01;43(12):2369-75. 2002-12-01 [PMID: 12613526] (Human)
• Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation. J. Virol., 2003-02-01;77(3):1727-37. 2003-02-01 [PMID: 12525606] (Primate - Macaca mulatta (Rhesus Macaque))
• Carstanjen D, Regenfus M, Muller C, Salama A Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase response. Exp. Hematol., 2001-07-01;29(7):812-21. 2001-07-01 [PMID: 11438203] (Human)
• Keller ET, 49536, Binkley NC, Stebler BA, Hall DM, Johnston GM, Zhang J, Ershler WB Ovariectomy does not induce osteopenia through interleukin-6 in rhesus monkeys (Macaca mulatta). CpG Type A Induction of an Early Protective Environment in Experimental Multiple Sclerosis, 2000-01-01;26(1):55-62. 2000-01-01 [PMID: 10617157] (Primate - Macaca mulatta (Rhesus Macaque))
• Hampel H, Teipel SJ, Padberg F, Haslinger A, Riemenschneider M, Schwarz MJ, Kotter HU, Scheloske M, Buch K, Stubner S, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ Discriminant power of combined cerebrospinal fluid tau protein and of the soluble interleukin-6 receptor complex in the diagnosis of Alzheimer's disease. Brain Res., 1999-03-27;823(1):104-12. 1999-03-27 [PMID: 10095017] (Human)
• Stubner S, Schon T, Padberg F, Teipel SJ, Schwarz MJ, Haslinger A, Buch K, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ, Hampel H Interleukin-6 and the soluble IL-6 receptor are decreased in cerebrospinal fluid of geriatric patients with major depression: no alteration of soluble gp130. Neurosci. Lett., 1999-01-15;259(3):145-8. 1999-01-15 [PMID: 10025579] (Human)

FAQs for IL-6R alpha (DR600). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL6R