Human Noggin ELISA Kit (Colorimetric)

ELISA: Human Noggin ELISA Kit (Colorimetric) [NBP2-80296] - Samples were spiked with high concentrations of Human Noggin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Noggin ELISA Kit (Colorimetric) [NBP2-80296] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Milk, Cell Supernatant, Tissue Homogenate
• Standard Curve Range: 125-8000 pg/mL
• Sensitivity: 75 pg/mL

Human Noggin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human NOG in samples. No significant cross-reactivity or interference between Human NOG and analogues was observed.
• Standard Curve Range: 125-8000 pg/mL
• Sensitivity: 75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.96%
• Intra-Assay: CV% < 5.37%
• Spike Recovery: 85-104%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NOG

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Noggin ELISA Kit (Colorimetric)

• NOG
• Noggin
• SYM1
• symphalangism 1 (proximal)
• synostoses (multiple) syndrome 1
• SYNS1
• SYNS1A

Background

Noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. The morphogenesis of organs is initiated by a downgrowth from a layer of epithelial stem cells. This process is achieved through the receipt of signals from 1) a WNT protein (WNT3A) to stabilize beta-catenin; and 2) Noggin, which is a bone morphogenetic protein inhibitor. Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have been identified, which have multiple joint fusion as their principal defect.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Noggin ELISA Kit (NBP2-80296). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NOG
• Uniprot:
  • Q13253()
  • Q13253()