Nodal Antibody

Western Blot: Nodal Antibody [NBP1-71686] - Analysis of NODAL in HeLa whole cell extracts.

Immunocytochemistry/Immunofluorescence: Nodal Antibody [NBP1-71686] - ICC staining of NODAL in HeLa cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.32 mg/ml

Nodal Antibody Summary

• Description: Novus Biologicals Rabbit Nodal Antibody (NBP1-71686) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A genomic peptide made to a C-terminal region of the human NODAL protein (within residues 200-347). [Swiss-Prot Q96S42]
• Localization: Secreted.
• Predicted Species: Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NODAL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500
• Application Notes: In Western blot, a band is seen ~40 kDa.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Immunogen has 98% identity to rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.1% BSA, and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.32 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Nodal Antibody

• BMP-16
• MGC138230
• nodal homolog (mouse)
• nodal homolog
• Nodal
• nodal, mouse, homolog

Background

NODAL is an important TGF-beta family protein in cellular differentiation. While constitutively expressed in many developing tissues, NODAL is problematically associated with the differentiation and development of many cancers. The expression of NODAL is regulated by a Notch signaling pathway, which when disrupted contributes to the aggressive behavior of metastatic cancer cells, particularly in melanoma. The abundance of NODAL expression in melanoma can help determine the stage of cancer and metastatic type/progression when tested in IHC. NODAL interactions with Cripto also predispose toward tumor progression. Overexpression of NODAL is also associated with preeclamptic placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NODAL
• Entrez:
  • 4838(Human)
  • 18119(Mouse)
• Uniprot:
  • P43021()
  • Q96S42()