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PITX2 Antibody [Unconjugated]

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PITX2 was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Sheep Anti-Human PITX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7388) at 3 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

PITX2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PITX2
Ala149-Val317
Accession # Q99697
Specificity
Detects human PITX2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human PITX1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PITX2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF7388 in the following applications:

  • ICC
    2 publications
  • IHC
    3 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PITX2 Antibody [Unconjugated]

  • all1-responsive gene 1
  • ALL1-responsive protein ARP1
  • ARP1MGC20144
  • Brx1
  • Homeobox protein PITX2
  • IDG2
  • IGDS
  • IGDS2
  • IHG2
  • IRID2
  • Otlx2
  • paired-like homeodomain 2
  • Paired-like homeodomain transcription factor 2MGC111022
  • pituitary homeo box 2
  • pituitary homeobox 2
  • PITX2
  • PTX2
  • RGS
  • RGSRIEG1Brx1
  • rieg bicoid-related homeobox transcription factor 1
  • RIEG bicoid-related homeobox transcription factor
  • RIEG
  • RIEG1
  • RP1
  • RS
  • Solurshin

Background

PITX2 (PITuitary homeoboX 2; also ARP1 and RIEG) is a 33-36 kDa nuclear member of the bicoid subfamily, paired homeobox family of transcription factors. It is widely expressed in the embryo, and serves as the earliest known marker for pituitary gland development. A long isoform commonly called PITX2C also plays a very key role in the establishment of left-right organ asymmetry. PITX2 appears in multiple germ layer derivatives, and it appears that one of its important functions is to integrating retinoic acid and Wnt/ beta -catenin signaling. The N- and C-terminal regions of PITX2 normally interact noncovalently, rendering PITX2 inactive.  Over aa 149‑317, human and mouse PITX2 are identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PITX2 Antibody (AF7388)(8)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Gecko.

We have publications tested in 2 applications: ICC, IHC.


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ICC
(2)
IHC
(3)
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Filter By Species
Human
(1)
Mouse
(3)
Rat
(1)
Gecko
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF7388 Applications Species
Su, D;Eliason, S;Sun, Z;Shao, F;Amendt, BA; Wolf-Hirschhorn Syndrome Candidate 1 (Whsc1) Methyltransferase Signals Via a Pitx2-miR-23/24 Axis to Effect Tooth Development The Journal of biological chemistry 2023-10-06 [PMID: 37806494] (IHC, Mouse) IHC Mouse
KS Brink, JI Henríquez, TM Grieco, JR Martin Del, K Fu, JM Richman Tooth Removal in the Leopard Gecko and the de novo Formation of Replacement Teeth Frontiers in Physiology, 2021-05-04;12(0):576816. 2021-05-04 [PMID: 34012403] (IHC, Gecko) IHC Gecko
YH Zhang, M Xu, X Shi, XL Sun, W Mu, H Wu, J Wang, S Li, P Su, L Gong, M He, M Yao, QF Wu Cascade diversification directs generation of neuronal diversity in the hypothalamus Cell Stem Cell, 2021-04-21;0(0):. 2021-04-21 [PMID: 33887179] (IHC, Mouse) IHC Mouse
Mason Sweat, Yan Sweat, Wenjie Yu, Dan Su, Riley J. Leonard, Steven L. Eliason et al. The miR-200 family is required for ectodermal organ development through the regulation of the epithelial stem cell niche Stem Cells 2021-06-01 [PMID: 33529466]
Gantner C, Cota-Coronado A, Thompson L, Parish C An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions STAR Protoc 10/28/2020 [PMID: 33111103]
Wenjie Yu, Zhao Sun, Yan Sweat, Mason Sweat, Shankar Rengasamy Venugopalan, Steven Eliason et al. Pitx2-Sox2-Lef-1 interactions specify progenitor oral/dental epithelial cell signaling centers Development 2020-01-01 [PMID: 32439755]
CW Gantner, IR de Luzy, JA Kauhausen, N Moriarty, JC Niclis, CR Bye, V Penna, CPJ Hunt, CM Ermine, CW Pouton, D Kirik, LH Thompson, CL Parish Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease Cell Stem Cell, 2020-02-13;0(0):. 2020-02-13 [PMID: 32059808] (ICC, Mouse, Rat) ICC Mouse, Rat
IR de Luzy, JC Niclis, CW Gantner, JA Kauhausen, CPJ Hunt, C Ermine, CW Pouton, LH Thompson, CL Parish Isolation of LMX1a ventral midbrain progenitors improves the safety and predictability of human pluripotent stem cell-derived neural transplants in Parkinsonian Disease J. Neurosci., 2019-10-22;0(0):. 2019-10-22 [PMID: 31641054] (ICC, Human) ICC Human

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Bioinformatics

Gene Symbol PITX2
Uniprot