HAI-2/SPINT2 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HAI-2/SPINT2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse HAI-2B
  Arg30-Lys140
  Accession # NP_001076017
• Specificity: Detects mouse HAI-2 Ectodomain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human HAI-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SPINT2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAI-2/SPINT2 Antibody [Unconjugated]

• HAI2
• HAI-2
• HAI-2DIAR3
• HAI2FLJ45571
• Hepatocyte growth factor activator inhibitor type 2
• Kop
• kunitz-type protease inhibitor 2
• PB
• Placental bikunin
• serine peptidase inhibitor, Kunitz type, 2
• serine protease inhibitor, Kunitz type, 2

Background

Two alternatively spliced forms of HAI-2 are found in human tissues. HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain. Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trypsin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa.

Three alternatively spliced forms of HAI-2 have been found in mouse tissues. HAI-2A is a minor form expressed in mouse tissues. HAI-2B, with residues 37 through 93 spliced out and thus lack of the first Kunitz domain, is a major form in mouse tissues. HAI-2B is an effective inhibitor of both HGF activator and trypsin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAI-2/SPINT2 Antibody (AF1107)(3)

Publications using AF1107

• CJ Wu, M Lu, X Feng, G Nakato, MC Udey Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins Cells, 2020;9(4):. 2020 [PMID: 32326212] (Western Blot, Mouse)
• Sales K, Friis S, Abusleme L, Moutsopoulos N, Bugge T Matriptase promotes inflammatory cell accumulation and progression of established epidermal tumors. Oncogene, 2015;34(35):4664-72. 2015 [PMID: 25486433] (Western Blot, Mouse)
• Szabo R, Hobson JP, List K, Molinolo A, Lin CY, Bugge TH Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity. J. Biol. Chem., 2008;283(43):29495-504. 2008 [PMID: 18713750] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SPINT2
• Uniprot:
  • NP_001076017()