NLRX1 Antibody - BSA Free

Western Blot: NLRX1 Antibody [NBP3-12566] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:108 kDaObserved band size:110 kDa

Immunohistochemistry-Paraffin: NLRX1 Antibody [NBP3-12566] - Staining of human NLRX1 in human urinary bladder with rabbit polyclonal antibody at 1:100 dilution.

Immunoprecipitation: NLRX1 Antibody [NBP3-12566] - Lane A:0.5 mg MCF7 Whole Cell Lysate4 uL anti-NLRX1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NLRX1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 108 kDaObserved band size :110 kDa

Immunocytochemistry/Immunofluorescence: NLRX1 Antibody [NBP3-12566] - Staining of NLRX1 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12566 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NLRX1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human NLRX1 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRX1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for NLRX1 Antibody - BSA Free

• Caterpiller protein 11.3
• CLR11.3DLNB26
• MGC131937
• MGC21025
• NLR family member X1
• NLR family, X1
• NOD26
• NOD5
• NOD9FLJ21478
• NOD-like receptor X1
• Nucleotide-binding oligomerization domain protein 26
• Nucleotide-binding oligomerization domain protein 5
• Nucleotide-binding oligomerization domain protein 9
• nucleotide-binding oligomerization domain, leucine rich repeat containing X1

Background

NLRX1 encodes a member of the NLR family. Alternative splicing has been observed at this gene locus and two transcript variants, encoding distinct isoforms, have been identified. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLRX1