![Western Blot: NLRC3 Antibody [NBP2-75593] - Analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Western-Blot-NBP2-75593-img0009.jpg "Western Blot: NLRC3 Antibody [NBP2-75593] - Analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit NLRC3 Antibody (NBP2-75593) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-NLRC3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Synthetic peptide within Human NLRC3 aa 1-50 / 1,065. (SwissProt: Q7RTR2 Human; SwissProt: Q5DU56 Mouse) |
| Localization | Cytoplasm. Cytoskeleton. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NLRC3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 120 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.2% BSA, 50% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
![Immunocytochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunocytochemistry__Immunofluorescence_21634.jpg)
![Immunohistochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunohistochemistry_9938.jpg)
| Publication using NBP2-75593 | Applications | Species |
|---|---|---|
| Kang JH, Li MJ, Luan PP et al. NLRC3 silencing accelerates the invasion of hepatocellular carcinoma cell via IL-6 induced JAK2/STAT3 pathway activation Cell Biol. Int. 2020-06-25 [PMID: 32584509] |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NLRC3 |
![Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75593-img0004.jpg "Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded mouse colon tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0008.jpg "Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded mouse colon tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: NLRC3 Antibody [NBP2-75593] - Analysis of MCF-7 cells with NLRC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Flow-Cytometry-NBP2-75593-img0001.jpg "Flow Cytometry: NLRC3 Antibody [NBP2-75593] - Analysis of MCF-7 cells with NLRC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75593-img0002.jpg "Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75593-img0003.jpg "Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human colon cancer tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0005.jpg "Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human colon cancer tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human spleen tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0006.jpg "Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human spleen tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human tonsil tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0007.jpg "Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human tonsil tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.")
![N/A CD25/IL-2R alpha [HRP]](https://images.novusbio.com/images2/IL-2_R_alpha_DR2A00_ELISA_167.jpg)
![N/A CD25/IL-2R alpha [HRP]](https://images.novusbio.com/images2/DATA_CD25_DR2A00_ELISA_800.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")