NLRX1 Antibody - Azide and BSA Free

Immunoprecipitation: NLRX1 Antibody [NBP2-93355] - Analysis of 600ug extracts of Mouse kidney using 3ug NLRX1 antibody. Western blot was performed from the immunoprecipitate using NLRX1 at a dilition of 1:1000.

Western Blot: NLRX1 Antibody - Azide and BSA Free [NBP2-93355] - Western blot analysis of lysates from Mouse heart, using NLRX1 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NLRX1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit NLRX1 Antibody - Azide and BSA Free (NBP2-93355) is a polyclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 87-360 of human NLRX1 (NP_078894.2). AEWFSRLPREERQFGPTFALDTVHVDPVIRESTPDELLRPPAELALEHQPPQAGLPPLALSQLFNPDACGRRVQTVVLYGTVGTGKSTLVRKMVLDWCYGRLPAFELLIPFSCEDLSSLGPAPASLCQLVAQRYTPLKEVLPLMAAAGSHLLFVLHGLEHLNLDFRLAGTGLCSDPEEPQEPAAIIVNLLRKYMLPQASILVTTRPSAIGRIPSKYVGRYGEICGFSDTNLQKLYFQLRLNQPYCGYAVGGSGVSATPAQRDHLVQMLSRNLEG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRX1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunoprecipitation 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 108 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for NLRX1 Antibody - Azide and BSA Free

• Caterpiller protein 11.3
• CLR11.3DLNB26
• MGC131937
• MGC21025
• NLR family member X1
• NLR family, X1
• NOD26
• NOD5
• NOD9FLJ21478
• NOD-like receptor X1
• Nucleotide-binding oligomerization domain protein 26
• Nucleotide-binding oligomerization domain protein 5
• Nucleotide-binding oligomerization domain protein 9
• nucleotide-binding oligomerization domain, leucine rich repeat containing X1

Background

NLRX1 encodes a member of the NLR family. Alternative splicing has been observed at this gene locus and two transcript variants, encoding distinct isoforms, have been identified. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: NLRX1