NLRX1 Antibody - BSA Free

Western Blot: NLRX1 Antibody [NBP1-76287] - Rat spleen tissue cell lysate with NOD5 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry: NLRX1 Antibody - BSA Free [NBP1-76287] - Immunohistochemistry of NLRX1 in rat spleen tissue with NLRX1 antibody at 10 u/mL.

Immunocytochemistry/ Immunofluorescence: NLRX1 Antibody - BSA Free [NBP1-76287] - Immunofluorescence of NLRX1 in rat spleen tissue with NLRX1 antibody at 20 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NLRX1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NLRX1 Antibody - BSA Free (NBP1-76287) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human NOD5. The immunogen is located within amino acids 70 - 120 of NOD5. Amino Acid Squence: FSRLPREERQFGPT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRX1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/mL
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-2 ug/ml
• Theoretical MW: 108 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  NLRX1 Peptide (NBP1-76287PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for NLRX1 Antibody - BSA Free

• Caterpiller protein 11.3
• CLR11.3DLNB26
• MGC131937
• MGC21025
• NLR family member X1
• NLR family, X1
• NOD26
• NOD5
• NOD9FLJ21478
• NOD-like receptor X1
• Nucleotide-binding oligomerization domain protein 26
• Nucleotide-binding oligomerization domain protein 5
• Nucleotide-binding oligomerization domain protein 9
• nucleotide-binding oligomerization domain, leucine rich repeat containing X1

Background

NOD5, also known as NLRX1, is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD5 localizes to the mitochondrial outer membrane and interacts with the virus-induced signaling adapter protein VISA. Unlike a subset of NOD-like receptors (NLRs) such as NOD1 and NOD2 which trigger pro-inflammatory cascades, and other NLRs that induce the caspase 1 inflammasome in response to immune challenges, NOD5 amplifies NF-kappaB and JNK pathways by inducing reactive oxygen species production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLRX1
• Entrez:
  • 79671(Human)
  • 270151(Mouse)
  • 315599(Rat)
• Uniprot:
  • Q86UT6(Human)