NLRP2/NALP2 Antibody (443625)

Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NLRP2/NALP2 Monoclonal Antibody (Catalog # MAB4684) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NLRP2/NALP2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clone: 443625
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NLRP2/NALP2 Antibody (443625) Summary

• Immunogen: E. coli-derived recombinant human NLRP2/NALP2
  Met1-Thr208
  Accession # Q9NX02
• Specificity: Detects human NLRP2/NALP2 in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NLRP2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NLRP2/NALP2 Antibody (443625)

• CLR19.9
• FLJ20510
• NACHT, leucine rich repeat and PYD containing 2
• NACHT, LRR and PYD domains-containing protein 2
• NALP2
• NALP2NACHT, LRR and PYD containing protein 2
• NBS1
• NBS1PYRIN-containing APAF1-like protein 2
• NLR family, pyrin domain containing 2
• NLRP2
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 2
• PAN1
• PAN1Nucleotide-binding site protein 1
• PYPAF2
• PYPAF2PYRIN domain and NACHT domain-containing protein 1

Background

NLRP2, also known as NALP2, is a cytosolic 121 kDa member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NF kappa B, it suppresses TNF‑ alpha production in response to LPS; via procaspase‑1, it promotes proIL‑1 beta cleavage and release. Human NLRP2 is 1062 amino acids (aa) in length. It contains an N‑terminal Pyrin domain (aa 9‑90), followed by a Nacht region and nine LRRs (aa 467‑1033). Four alternate isoforms exist. Three show deletions of aa 109‑132, aa 133‑154 and aa 847‑1062, respectively; a fourth shows a 38 aa substitution for aa 1‑903. No close rodent counterparts have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NLRP2/NALP2 Antibody (MAB4684)(2)

Publications using MAB4684

• László Ducza, Péter Szücs, Krisztina Hegedűs, Erzsébet Bakk, Andrea Gajtkó, Ildikó Wéber et al. NLRP2 Is Overexpressed in Spinal Astrocytes at the Peak of Mechanical Pain Sensitivity during Complete Freund Adjuvant-Induced Persistent Pain International Journal of Molecular Sciences 2021-10-22 [PMID: 34768839]
• László Ducza, Péter Szücs, Krisztina Hegedűs, Erzsébet Bakk, Andrea Gajtkó, Ildikó Wéber et al. NLRP2 Is Overexpressed in Spinal Astrocytes at the Peak of Mechanical Pain Sensitivity during Complete Freund Adjuvant-Induced Persistent Pain International Journal of Molecular Sciences 2021-10-22 [PMID: 34768839] (Immunohistochemistry, Western Blot, Rat)

Bioinformatics

• Gene Symbol: NLRP2
• Uniprot:
  • Q9NX02()