NLRP1/NALP1 Antibody

Images

 
Immunohistochemistry: NLRP1/NALP1 Antibody [NB100-56148] - OHT-induced upregulation of Aim2 inflammasome in the retina. NLRP1 antibody labeling (red) in the inner retina; yellow arrows indicate colocalization with RGC ...read more
Western Blot: NLRP1/NALP1 Antibody [NB100-56148] - Analysis of NLRP1/NALP1 in adult human tissue lysates using NLRP1 antibody at 1:2000. Full-length NLRP1 protein was detected at highest levels in kidney, brain and ...read more
Immunohistochemistry: NLRP1/NALP1 Antibody [NB100-56148] - The NLRP1and NLRP3 proteins localized to distinct cell types in the GCL of naive and NT control retinas: NLRP3 (green) is only expressed in blood vessels (white ...read more
Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56148] - FFPE normal human tissue sections stained for NALP1 (CARD7/NAC) using NB100-56148 at 1:2000. (A) epidermis, (B) esophagus (C) uterine cervix, and (D) ...read more
Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56148] - FFPE human tissue sections stained for NALP1 (CARD7/NAC) using NB100-56148 at 1:2000. (A) bone marrow and (C) brain glioma core from a brain cancer ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NLRP1/NALP1 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 161-180 (PSSPDHESPSQESPNAPTST) of human NLRP1/NALP1 was used as immunogen, GenBank no. NP_127497.1.
Specificity
NALP1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NLRP1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
Control
NLRP1/NALP1 Overexpression Lysate
Publications
Read Publication using
NB100-56148 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30930743).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for NLRP1/NALP1 Antibody

  • CARD7
  • CARD7SLEV1
  • Caspase recruitment domain-containing protein 7
  • CLR17.1
  • Death effector filament-forming ced-4-like apoptosis protein
  • DEFCAP
  • DEFCAPVAMAS1
  • DKFZp586O1822
  • KIAA0926DEFCAP-L/S
  • NACHT, leucine rich repeat and PYD (pyrin domain) containing 1
  • NACHT, leucine rich repeat and PYD containing 1
  • NACHT, LRR and PYD containing protein 1
  • NACHT, LRR and PYD domains-containing protein 1
  • NACPP1044
  • NALP1
  • NALP1caspase recruitment domain protein 7
  • NLR family, pyrin domain containing 1
  • NLRP1
  • Nucleotide-binding domain and caspase recruitment domain
  • nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 1
  • PP1044
  • SLEV1
  • VAMAS1

Background

NALP1 (also known as NAC/CARD7) is a member of the CATERPILLER (CLR) diverse multigene superfamily of immune regulatory proteins (Ting and Davis, 2005). CLR proteins have a variable N-terminal domain, followed by a nucleotide-binding domain (NBD) and leucine-rich repeats (LRR). The N-terminal domain consists of transactivation, CARD, Pyrin or BIR domains, or in some cases is undefined. The CLR family, also known as the NOD family, has its ancient roots in the plant kingdom and is related to the disease resistance (R) gene family that mediates plant immune responses. CLR proteins participate in both innate and adaptive immunity in mammals, some act as sensors that detect pathogen products, and several CLR genes have been linked to susceptibility to immunological diseases. It is thought that the CLR family may have a fundamental importance for the function of the mammalian innate/ancient immune system on par with the Toll-like receptor (TLR) family. NALP1, a member of NALP CLR subfamily, has an N-terminal pyrin domain (PYD), followed by an NBD, LRR, and a C-terminal CARD domain (reviewed in Tschopp. NALP1 is a component of both the inflammasome and apoptosome, suggesting that NALP1 it has important roles in both inflammation and apoptosis. This antibody recognizes NALP1; human NALP1 is a 1473 amino acid protein and migrates at approx. 166 kDa on SDS-PAGE. However, multiple splice variants encoding distinct NALP1 isoforms with varying amino acid lengths have been described and thus the molecular weight detected may vary depending on the isoform(s) expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NB100-56708
Species: Ch, ChHa, Hu, Ma, Mu, Po, Rt
Applications: CyTOF-ready, EM, Flow, HEStain, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, KD, KO, Simple Western, WB
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
MAB17761
Species: Hu, Mu, Rt
Applications: ICC, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-67471
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-45626
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-24915
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
D6050
Species: Hu
Applications: ELISA
NBP2-27335
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NB100-56503
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
NB300-605
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NBP2-12446
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IM, IP, KD, WB
DY3667
Species: Mu
Applications: ELISA
NB100-56148
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Supplier Logo

Publications for NLRP1/NALP1 Antibody (NB100-56148)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-Fr.


Filter By Application
IHC-Fr
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for NLRP1/NALP1 Antibody (NB100-56148) (0)

There are no reviews for NLRP1/NALP1 Antibody (NB100-56148). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NLRP1/NALP1 Antibody (NB100-56148) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional NLRP1/NALP1 Products

Bioinformatics Tool for NLRP1/NALP1 Antibody (NB100-56148)

Discover related pathways, diseases and genes to NLRP1/NALP1 Antibody (NB100-56148). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NLRP1/NALP1 Antibody (NB100-56148)

Discover more about diseases related to NLRP1/NALP1 Antibody (NB100-56148).
 

Pathways for NLRP1/NALP1 Antibody (NB100-56148)

View related products by pathway.

PTMs for NLRP1/NALP1 Antibody (NB100-56148)

Learn more about PTMs related to NLRP1/NALP1 Antibody (NB100-56148).
 

Research Areas for NLRP1/NALP1 Antibody (NB100-56148)

Find related products by research area.

Blogs on NLRP1/NALP1.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NLRP1/NALP1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NLRP1
Entrez